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The expression of thermoTRP channels in thebrood patch of jungle fowl (Gallus gallus) during
Linköping University, Department of Physics, Chemistry and Biology.
2009 (English)Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Abstract [en]

The regulation of egg temperature requires the transfer of heat from the brood patch. Thus, the brood patch needs the presence of thermo receptors as well as an appropriate vasomotor response. During the incubation an exact detection of the egg's temperature is essential. So, in this study we attempted to detect the presence and regulation of thermo TRP channels (thermo Transient Receptor Potential channels) (TRPV1, TRPV3, TRPV4, TRPM8 and TRPA1) expressions during egg incubation. Six incubating Jungle fowl hens, and five non incubating jungle fowl hens and one jungle fowl cock were used as main samples and controls. Total RNA was extracted from liver, kidney, heart, blood, White Blood Cell, Dorsal Root Ganglion and skin. The samples from the skin were taken from the brood patch and inter scapular region. PCR investigation showed that different thermo TRP channels were expressed in different tissues. TRPV1, V3, V4 and M8 mRNA were detected in the skin of brood patch. However, V1 and V3 expression in the brood patch skin did not differ between broody and non broody hens. In conclusion, although considerable morphological changes in the skin of brood patch could be seen, the expression of TRPV1 and V3 channels did not change significantly, but this cannot exclude the alteration in the expression of TRP channels in different stages of broodiness or specific parts of skin like AVAs (Arteriovenous anastomosis) which will be the subject for more studies.

Place, publisher, year, edition, pages
2009. , 20 p.
Keyword [en]
Brood patch, broody, jungle fowl, skin, thermoTRP
National Category
URN: urn:nbn:se:liu:diva-19563ISRN: LITH-IFM-A-EX--—09/2130—SEOAI: diva2:225426
Subject / course
Molecular Biotechnology
Schrödinger, Physics building, Linköping University (English)
Life Earth Science
Available from: 2009-07-08 Created: 2009-06-26 Last updated: 2011-07-08Bibliographically approved

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Jafari, Shadi
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Department of Physics, Chemistry and Biology

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