Gene Expression Analysis of Adipogenic, Chondrogenic and Osteogenic Induced Human Dermal Fibroblasts
(English)Manuscript (preprint) (Other academic)
The use of adult stem cells in tissue engineering applications is a promising alternativewhen the possibility of acquiring autologous cells for transplantation is limited. Even so, theadult stem cell populations identified up to this point are far from optimal in aspects ofharvest and culture expansion. With the recent suggestion of stem cell plasticity inherent inhuman dermal fibroblasts, a new plausible candidate for use as cell source in tissuereconstruction has emerged. Fibroblast cultures can be induced to differentiate towardsadipocyte, chondrocyte and osteoblast-like cells in vitro, by the use of induction media. Thepresent works utilizes Affymetrix full expression micro array to identify if genes commonlyexpressed in stem cells differentiating towards the above-mentioned lineages also areexpressed in induced fibroblasts. Several genes important for differentiation andmaintenance of an adipose, cartilage or bone phenotype were found up-regulated in theinduced cultures. The results presented here provide further evidence for the plasticity ofhuman dermal fibroblasts, and their possible use in tissue engineering and reconstructivesurgery.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-19715OAI: oai:DiVA.org:liu-19715DiVA: diva2:227692