liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gaskromatografisk metod för analys av GHB i urin
Linköping University, Department of Physics, Chemistry and Biology.
2009 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
Gas chromatographic method for GHB analysis in urine (English)
Abstract [sv]

En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer. Detektion ner till 100 μmol/L är möjligt med en variationskoefficient mellan 6 och 12 %. Provsvar erhålls efter 6,5 minuter. Metoden är dock inte fullständig då en del frågetecken kvarstår. Bland annat bör det undersökas om andra föreningar, som kan förekomma i urin, kan eluera samtidigt som GHB. Om ja så bör vidare analyser genomföras för att separera GHB och den andra föreningen. Metoden kan däremot användas i nuläget som en screeninganalys för att snabbt få ett svar på om GHB finns närvarande eller inte. Verifiering kan sedan ske med GC-MS.

Abstract [en]

A method for determination and quantification of gamma-hydroxyburyric acid (GHB) in urine samples is developed at Sahlgrenska universitetssjukhus. No time consuming procedures as derivatization and exctration is required, which makes the method fairly unique. Hydrochloric acid and internal standard, gamma-valerolakton, is added to the urine sample before the sample is injected to a gas chromatograph with a flame ionization detector and a column for glycols and alcohols. The hydrochloric acid makes the GHB convert into gamma-butyrolactone (GBL) which is easier to separate in the gas chromatograph. Limit of detection was found to be 100 μmol/L and test result is received after 6,5 minutes. There are still some question marks around the method, for example, there is a possibility that another substance elute at the same time as GHB. More tests are required to determine whether or not it is so. For now the method can be used as a screening analysis to hastily detect GHB presence. Verification can be done with GC-MS.

Place, publisher, year, edition, pages
2009. , 29 p.
Keyword [en]
gas chromatography, gamma-hydroxybutyric acid, urine
Keyword [sv]
GHB, gamma-hydroxybutyrat, gamma-hydroxysmörsyra, gaskromatografi, GC, urin, urinprov
National Category
Other Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-19651ISRN: LITH-IFM-G-EX--09/2083--SEOAI: oai:DiVA.org:liu-19651DiVA: diva2:227916
Subject / course
Chemical Engineering
Presentation
2009-06-03, 10:20 (Swedish)
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2009-08-11 Created: 2009-07-10 Last updated: 2011-07-08Bibliographically approved

Open Access in DiVA

fulltext(1237 kB)1534 downloads
File information
File name FULLTEXT01.pdfFile size 1237 kBChecksum SHA-512
2ea5917ba08f4b39c5d55ad31e16d84b65222a942abdc3301891258d9ec7fc41cdc6f5395f17e53a2e4788ad6cfadcda1d4092f44d86e73ffbd4e21915cc8e8f
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Jansson, Emelie
By organisation
Department of Physics, Chemistry and Biology
Other Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 1534 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 393 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf