β-tubulin mutations in ovarian cancer using single strand conformation analysis – risk of false positive results from paraffin embedded tissues
2006 (English)In: Cancer Letters, ISSN 0304-3835, Vol. 236, no 1, 148-154 p.Article in journal (Refereed) Published
Mutations in the β-tubulin gene have been proposed as a resistance mechanism to paclitaxel. We therefore investigated the presence of mutations in the β-tubulin M40 gene in 40 ovarian tumours (16 paraffin-embedded and 24 freshly frozen) selected for good or poor response to chemotherapy with paclitaxel or non-tubulin-affecting regimens. The presence of mutations was investigated using single strand conformation analysis followed by sequencing of the products with altered mobility. No sequence variants in the exons of the β-tubulin M40 gene were detected. Non-reproducible shifts were identified, in eight out of 16 paraffin embedded samples. This may explain some of the previously published discrepancies. In conclusion, sequence variants in the β-tubulin M40 gene are rare and are unlikely to be a clinically relevant explanation of resistance to paclitaxel.
Place, publisher, year, edition, pages
2006. Vol. 236, no 1, 148-154 p.
β-tubulin; Paclitaxel; Ovarian cancer; Mutation analysis; SSCA
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-14242DOI: 10.1016/j.canlet.2005.05.025OAI: oai:DiVA.org:liu-14242DiVA: diva2:22991
Henrik Green, Per Rosenberg, Peter Söderkvist, György Horvath and Curt Peterson, β-tubulin mutations in ovarian cancer using single strand conformation analysis – risk of false positive results from paraffin embedded tissues, 2006, Cancer Letters, (236), 1, 148-154.
Copyright: Elsevier Science B.V., Amsterdam.