liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A new route to the formation of biomimetic phosphate assemblies on gold: Synthesis and characterization
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics . Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics . Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics . Linköping University, The Institute of Technology.
Show others and affiliations
2006 (English)In: Journal of Colloid and Interface Science, ISSN 1095-7103, Vol. 295, no 1, 41-49 p.Article in journal (Refereed) Published
Abstract [en]

A biomimetic model system based on long-chain alkanethiols tailored with serine, threonine and tyrosine side-chain groups is created as a platform for the study of phosphorylated amino acids. The phosphorylated analogues are synthesized with protective tert-butyl groups that after assembly on thin polycrystalline gold films are removed in an acidic deprotection solution to form the corresponding phosphate self-assembled monolayers (SAMs). The SAMs are thoroughly characterized with null ellipsometry, contact angle goniometry, infrared reflection–absorption spectroscopy and X-ray photoelectron spectroscopy. The assembly and the subsequent deprotection process are optimized with respect to molecular orientation and chain conformation by varying the incubation time and the exposure time to the deprotection solution. The high quality of the generated SAMs suggests that the present assembly/deprotection approach is an attractive alternative when traditional synthetic routes become demanding because of solubility problems.

Place, publisher, year, edition, pages
2006. Vol. 295, no 1, 41-49 p.
Keyword [en]
SAM; Thiols; Gold; Phosphorylated amino acids; Surface deprotection scheme
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-14334DOI: 10.1016/j.jcis.2005.08.026OAI: oai:DiVA.org:liu-14334DiVA: diva2:23263
Available from: 2007-03-16 Created: 2007-03-16 Last updated: 2010-09-06
In thesis
1. Biomimetic surfaces: Preparation, characterization and application
Open this publication in new window or tab >>Biomimetic surfaces: Preparation, characterization and application
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [sv]

I denna avhandling beskrivs tillverkning, karaktärisering och tillämpning av ett antal biomimetiska ytor. Biomimetik är att härma naturen och grundtanken är att titta på hur naturen löst liknande problemställningar. Två olika typer av modellsystem med inspiration från naturen har tagits fram för framtida tillämpningar inom bioanalys, biosensorer samt antifrysmaterial. Det ena typen av modellsystem innefattar fosforylerade ytor och det andra består av ytor som härmar antifrys(glyko)proteiner. Ytorna tillverkades av monolager av självorganiserande svavelorganiska molekyler och karaktäriserades före tillämpning med hjälp av ellipsometri, IR-spektroskopi, kontaktvinkelmätning och röntgenfotoelektronspektroskopi.

Modellsystemen för att studera vattenfrysning på ytor inspirerades av antifrys(glyko)proteiner som bl.a. kan hittas i polarfiskar. Två modellsystem utvecklades och studerades med avseende på frysning av kondenserat vatten. Det ena designades att härma den aktiva domänen hos ett antifrysglykoproteiner (AFGP) och det andra härmade typ I antifrysproteiner (AFP I). Frysstudierna visade på signifi-kanta skillnader för AFGP-modellen jämfört med ett (OH/CH3) referenssystem med jämförbar vätbarhet, men inte för AFP Imodellen. Vattnet frös vid högre temperatur för AFGPmodellen.

Modellsystemen med fosforylerade ytor inspirerades av fosforylering och biomineralisering. Två system utvecklades, ett med långa och ett med korta alkylkedjor på aminosyraanalogerna, både med och utan fosfatgrupp. En ny metod användes med skyddsgrupper på fosfaterna hos de långa analogerna innan bildandet av monolager. Skyddsgrupperna togs bort efter bildandet av monolager. Dessa monolager undersöktes också med elektrokemiska metoder och signifikant högre kapacitans observerades för de fosforylerade monolageren jämfört med de icke fosforylerade.

Abstract [en]

This thesis describes the preparation, characterization and application of a few biomimetic surfaces. Biomimetics is a modern development of the ancient Greek concept of mimesis, i.e. man-made imitation of nature. The emphasis has been on the preparation and characterization of two types of model systems with properties inspired by nature with future applications in bioanalysis, biosensors and antifreeze materials. One type of model system involves phosphorylated surfaces; the other consists of surfaces mimicking antifreeze (glyco)proteins. The surfaces were made by chemisorbing organosulfur substances to a gold surface into monomolecular layers, so called self-assembled monolayers (SAMs). The physicochemical properties of the SAMs were thoroughly characterized with null ellipsometry, contact angle goniometry, x-ray photoelectron spectroscopy and infrared spectroscopy prior to application.

The work on antifreeze surfaces was inspired by the structural properties of antifreeze (glyco)proteins, which can be found in polar fish. Two model systems were developed and studied with respect to ice nucleation of condensed water layers. One was designed to mimic the active domain of antifreeze glycoproteins (AFGP) and the other mimicked type I antifreeze proteins (AFP I). Subsequent ice nucleation studies showed a significant difference between the AFGP model and a (OH/CH3) reference system displaying identical wetting properties, whereas the AFP I model was indistinguishable from the reference system.

The model systems with phosphorylated surfaces were inspired from phosphorylations and biomineralization. Two systems were developed, short- and long-chained amino acid analogues, with and without a phosphate group. A novel approach with protected groups before attachment to gold were developed for the long-chained analogues. The protective groups could be removed successfully after assembly. The long-chained SAMs were evaluated with electrochemical methods and significantly higher capacitance values were observed for the phosphorylated SAMs compared to the non-phosphorylated.

Place, publisher, year, edition, pages
Institutionen för fysik, kemi och biologi, 2007. 84 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1069
Keyword
Self-assembled monolayers, biomimetic surfaces, phosphates, antifreeze, surface spectroscopy
National Category
Atom and Molecular Physics and Optics
Identifiers
urn:nbn:se:liu:diva-8492 (URN)978-91-85715-99-2 (ISBN)
Public defence
2007-03-30, Planck, Fysikhuset, Campus Valla, Linköping, 10:15 (English)
Opponent
Supervisors
Available from: 2007-03-16 Created: 2007-03-16 Last updated: 2009-04-23

Open Access in DiVA

No full text

Other links

Publisher's full textLink to Ph.D. thesis

Authority records BETA

Borgh, AnnikaEkeroth, JohanPetoral Jr., Rodrigo M.Uvdal, KajsaKonradsson, PeterLiedberg, Bo

Search in DiVA

By author/editor
Borgh, AnnikaEkeroth, JohanPetoral Jr., Rodrigo M.Uvdal, KajsaKonradsson, PeterLiedberg, Bo
By organisation
Sensor Science and Molecular Physics The Institute of TechnologyDepartment of Physics, Chemistry and BiologyOrganic Chemistry
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 749 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf