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Electrochemical Evaluation of the Interfacial Capacitance upon Phosphorylation of Amino Acid Analogue Molecular Films
Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics . Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics . Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
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2001 (English)In: Analytical Chemistry, ISSN 0003-2700, Vol. 73, no 18, 4463-4468 p.Article in journal (Refereed) Published
Abstract [en]

An approach based on electrochemistry to differentiate between phosphorylated and nonphosphorylated amino acid analogues adsorbed on gold is presented. Analogues of serine, threonine, and tyrosine, containing thiohexadecyl headgroups, were synthesized and assembled on gold, and the surface capacitance was evaluated using electrochemical impedance spectroscopy. A procedure for deprotection of tert-butyl phosphate protecting groups, on the monolayer, is also described. Characterizations of the assembled analogues by cyclic voltammetry, infrared spectroscopy, and ellipsometry are used to confirm the insulating properties of the monolayers and the outcome of surface modifications. The results from cyclic voltammetry show good insulating properties for the monolayers even after phosphate deprotection. The infrared measurements reveal well-ordered monolayers, and the thickness from ellipsometry is in good agreement with expectations from molecular modeling. The impedance experiments show a capacitance increase up to 0.6 μF/cm2 as phosphate groups are introduced. The results in this study indicate the possibility of using a surface chemical and impedance spectroscopy approach to detect the kinase/phosphatase activity and kinetics involved in phosphorylation reactions.

Place, publisher, year, edition, pages
2001. Vol. 73, no 18, 4463-4468 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-14335DOI: 10.1021/ac010487+OAI: diva2:23264
Available from: 2007-03-16 Created: 2007-03-16 Last updated: 2009-05-11
In thesis
1. Biomimetic surfaces: Preparation, characterization and application
Open this publication in new window or tab >>Biomimetic surfaces: Preparation, characterization and application
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [sv]

I denna avhandling beskrivs tillverkning, karaktärisering och tillämpning av ett antal biomimetiska ytor. Biomimetik är att härma naturen och grundtanken är att titta på hur naturen löst liknande problemställningar. Två olika typer av modellsystem med inspiration från naturen har tagits fram för framtida tillämpningar inom bioanalys, biosensorer samt antifrysmaterial. Det ena typen av modellsystem innefattar fosforylerade ytor och det andra består av ytor som härmar antifrys(glyko)proteiner. Ytorna tillverkades av monolager av självorganiserande svavelorganiska molekyler och karaktäriserades före tillämpning med hjälp av ellipsometri, IR-spektroskopi, kontaktvinkelmätning och röntgenfotoelektronspektroskopi.

Modellsystemen för att studera vattenfrysning på ytor inspirerades av antifrys(glyko)proteiner som bl.a. kan hittas i polarfiskar. Två modellsystem utvecklades och studerades med avseende på frysning av kondenserat vatten. Det ena designades att härma den aktiva domänen hos ett antifrysglykoproteiner (AFGP) och det andra härmade typ I antifrysproteiner (AFP I). Frysstudierna visade på signifi-kanta skillnader för AFGP-modellen jämfört med ett (OH/CH3) referenssystem med jämförbar vätbarhet, men inte för AFP Imodellen. Vattnet frös vid högre temperatur för AFGPmodellen.

Modellsystemen med fosforylerade ytor inspirerades av fosforylering och biomineralisering. Två system utvecklades, ett med långa och ett med korta alkylkedjor på aminosyraanalogerna, både med och utan fosfatgrupp. En ny metod användes med skyddsgrupper på fosfaterna hos de långa analogerna innan bildandet av monolager. Skyddsgrupperna togs bort efter bildandet av monolager. Dessa monolager undersöktes också med elektrokemiska metoder och signifikant högre kapacitans observerades för de fosforylerade monolageren jämfört med de icke fosforylerade.

Abstract [en]

This thesis describes the preparation, characterization and application of a few biomimetic surfaces. Biomimetics is a modern development of the ancient Greek concept of mimesis, i.e. man-made imitation of nature. The emphasis has been on the preparation and characterization of two types of model systems with properties inspired by nature with future applications in bioanalysis, biosensors and antifreeze materials. One type of model system involves phosphorylated surfaces; the other consists of surfaces mimicking antifreeze (glyco)proteins. The surfaces were made by chemisorbing organosulfur substances to a gold surface into monomolecular layers, so called self-assembled monolayers (SAMs). The physicochemical properties of the SAMs were thoroughly characterized with null ellipsometry, contact angle goniometry, x-ray photoelectron spectroscopy and infrared spectroscopy prior to application.

The work on antifreeze surfaces was inspired by the structural properties of antifreeze (glyco)proteins, which can be found in polar fish. Two model systems were developed and studied with respect to ice nucleation of condensed water layers. One was designed to mimic the active domain of antifreeze glycoproteins (AFGP) and the other mimicked type I antifreeze proteins (AFP I). Subsequent ice nucleation studies showed a significant difference between the AFGP model and a (OH/CH3) reference system displaying identical wetting properties, whereas the AFP I model was indistinguishable from the reference system.

The model systems with phosphorylated surfaces were inspired from phosphorylations and biomineralization. Two systems were developed, short- and long-chained amino acid analogues, with and without a phosphate group. A novel approach with protected groups before attachment to gold were developed for the long-chained analogues. The protective groups could be removed successfully after assembly. The long-chained SAMs were evaluated with electrochemical methods and significantly higher capacitance values were observed for the phosphorylated SAMs compared to the non-phosphorylated.

Place, publisher, year, edition, pages
Institutionen för fysik, kemi och biologi, 2007. 84 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1069
Self-assembled monolayers, biomimetic surfaces, phosphates, antifreeze, surface spectroscopy
National Category
Atom and Molecular Physics and Optics
urn:nbn:se:liu:diva-8492 (URN)978-91-85715-99-2 (ISBN)
Public defence
2007-03-30, Planck, Fysikhuset, Campus Valla, Linköping, 10:15 (English)
Available from: 2007-03-16 Created: 2007-03-16 Last updated: 2009-04-23

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Ekeroth, JohanBjörefors, FredrikBorgh, AnnikaLundström, IngemarLiedberg, BoKonradsson, Peter
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