Alpha-1-acid glycoprotein interacts with the neutrophilproteins S100A8 and S100A9
(English)Manuscript (preprint) (Other academic)
The acute phase protein α1-acid glycoprotein (AGP) has been indicated tobind to neutrophils and to affect neutrophil functions. In the presentinvestigation neutrophil proteins interacting with AGP was studied. Twolow molecular weight proteins were isolated from neutrophil lysates byaffinity chromatography on a column with immobilized AGP. The proteinswere identified as the calcium-binding, myeloid related proteins S100A8and S100A9 using mass spectrometry and Western blot analyses. Theinteraction was further confirmed using a biotin affinity-tagging procedure.The interaction between AGP and S100A8/A9 was sensitive to EDTAindicating a calcium-dependent binding. Desialylation andhyperfucosylation of AGP did not affect its binding to S100A8/A9.
Neutrophils, plasma proteins, inflammation, affinity chromatography
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-20266OAI: oai:DiVA.org:liu-20266DiVA: diva2:233556