Peripheral Lipopolysaccharide Administration Induces Cytokine mRNA Expression in the Viscera and Brain of Fever-Refractory Mice Lacking Microsomal Prostaglandin E Synthase-1
2009 (English)In: Journal of neuroendocrinology (Print), ISSN 0953-8194, E-ISSN 1365-2826, Vol. 21, no 8, 715-721 p.Article in journal (Refereed) Published
We examined the expression of interleukin (IL)-1 beta, IL-6 and tumour necrosis factor (TNF) alpha in mice lacking microsomal prostaglandin E synthase-1 (mPGES-1), which neither produce prostaglandin E-2, nor mount a febrile response upon immune challenge. Intraperitoneal lipopolysaccharide (LPS) injection resulted in a strongly induced expression of all three cytokines in the brain and viscera, similar to wild-type animals. Several brain regions additionally showed modest induction of receptors for these cytokines in both genotypes. Telemetric recordings of body temperature showed that the mPGES-1 deficient mice remained afebrile upon LPS challenge, in contrast to the prominent fever displayed by the wild-type mice. These data demonstrate that LPS-induced cytokine expression occurs independently of prostaglandin E-2, and imply that endogenously expressed IL-1 beta, IL-6, and TNF alpha are not pyrogenic per se, supporting the role of prostaglandin E-2 as the final and obligatory mediator of LPS-induced fever.
Place, publisher, year, edition, pages
2009. Vol. 21, no 8, 715-721 p.
Fever; prostaglandin E-2; interleukin-6; interleukin-1 beta; tumour necrosis factor alpha
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-20165DOI: 10.1111/j.1365-2826.2009.01888.xOAI: oai:DiVA.org:liu-20165DiVA: diva2:233676