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Electrochemical modulation of epithelia formation using conducting polymers
Karolinska Institutet.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-2071-7768
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.ORCID iD: 0000-0003-4791-4785
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2009 (English)In: Biomaterials, ISSN 0142-9612, Vol. 30, no 31, 6257-6264 p.Article in journal (Refereed) Published
Abstract [en]

Conducting polymers are soft, flexible materials, exhibiting material properties that can be reversibly changed by electrochemically altering the redox state. Surface chemistry is an important determinant for the molecular events of cell adhesion. Therefore, we analyzed whether the redox state of the conducting polymer PEDOT:Tosylate can be used to control epithelial cell adhesion and proliferation. A functionalized cell culture dish comprising two adjacent electrode surfaces was developed. Upon electronic addressing, reduced and oxidized surfaces are created within the same device. Simultaneous analysis of how a homogenous epithelial MDCK cell population responded to the electrodes revealed distinct surface-specific differences. Presentation of functional fibronectin on the reduced electrode promoted focal adhesion formation, involving αvβ3 integrin, cell proliferation, and ensuing formation of polarized monolayers. In contrast, the oxidized surface harbored only few cells with deranged morphology showing no indication of proliferation. This stems from the altered fibronectin conformation, induced by the different surface chemistry of the PEDOT:Tosylate electrode in the oxidized state. Our results demonstrate a novel use of PEDOT:Tosylate as a cell-hosting material in multiple-electrode systems, where cell adhesion and proliferation can be controlled by electrochemical modulation of surface properties.

Place, publisher, year, edition, pages
2009. Vol. 30, no 31, 6257-6264 p.
Keyword [en]
Actin; Cell adhesion; Electroactive polymer; Epithelial cell; Fibronectin; RGD peptide
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-20545DOI: 10.1016/j.biomaterials.2009.07.059OAI: diva2:234977
Original Publication: Karl Svennersten, Maria H. Bolin, Edwin W.H. Jager, Magnus Berggren and Agneta Richter-Dahlfors, Electrochemical modulation of epithelia formation using conducting polymers, 2009, Biomaterials, (30), 31, 6257-6264. Copyright: Elsevier Science B.V., Amsterdam. Available from: 2009-09-11 Created: 2009-09-11 Last updated: 2015-05-06
In thesis
1. Conjugated Polymer Surface Switches for Active Control
Open this publication in new window or tab >>Conjugated Polymer Surface Switches for Active Control
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polymers have been found useful in a wide range of applications such as sensors, electrochemical transistors, solar cells, and printed electronics due to their mechanical, optical and electronic properties. An amazing research field has grown during the last three decades since the discovery of conducting polymers in 1976. Since the materials can be made from solutions, different processing methods such as spin coating and vapor phase polymerization can be used to coat a huge variety of substrates. The choice of method depends mainly on monomer solubility and kind of substrate to be coated. During the synthesis the polymers can be chemically modified to tailor their functionalities. Due to this variability in materials and the processability, electronics can be achieved on unconventional substrates such as flexible plastic foils and cell culturing dishes. As a contrast to inorganic, usually metallic materials, conducting polymers are built up from organic compounds in a molecular structure with soft mechanical properties that have shown to be a benefit in combination with biology, ranging from interactions with cells to interactions with advanced biological species such as tissues. This combination of research fields and the possible applications are merged within the field of organic bioelectronics.

The primary purpose of this thesis is to give a background to organic electronics in general and how electrochemical devices can be processed and developed for biological applications in particular. An organic electronic surface switch is introduced to control cell adhesion and proliferation as well as an electrochemical transistor to spatially tune the cell adhesion along an electrochemical gradient. To mimic a more natural cell environment a three dimensional fiber substrate was used to design an electronically active matrix to promote nerve cell adhesion and communication. By combining standard microfabrication techniques and conjugated polymers desired patterns of electroactive polymer were created to enable active regulation of cell populations and their extracellular environment at high spatial resolution. Finally, a brief look into future challenges will also be presented.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 52 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1398
National Category
Natural Sciences
urn:nbn:se:liu:diva-71361 (URN)978-91-7393-063-5 (ISBN)
Public defence
2011-10-21, K3, Kåkenhus, Campus Norrköping, Linköpings universitet, Norrköping, 13:15 (English)
Available from: 2011-10-13 Created: 2011-10-13 Last updated: 2015-05-06Bibliographically approved

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