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Anti-insulin activity in IgG-fractions from children with newly-diagnosed type 1 diabetes and negative for insulin autoantibodies
Avd för Molekylär Medicin Helsingfors, Finland.
Barn och Ungdomssjukhuset Helsingfors, Finland.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Pediatrics. Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping.
2004 (English)In: Autoimmunity, ISSN 0891-6934, Vol. 37, no 1, 45-49 p.Article in journal (Refereed) Published
Abstract [en]

Insulin autoantibodies (IAA) are often detected as the first humoral sign of β-cell autoimmunity in prospective studies in young children with increased genetic risk of type 1 diabetes. After the appearance of IAA their level typically rise but seems to decline in many cases before the clinical presentation of type 1 diabetes. We hypothesized that the reason for the sudden drops in the levels of IAA could be the formation of immune complexes caused by binding of antibodies to free insulin in plasma. We studied whether isolation of the IgG-fraction and dissociation of immune complexes by acid treatment using protein A column results in the appearance of detectable IAA in those children with newly-diagnosed type 1 diabetes whose plasma samples test negative for IAA. IAA assay was performed in IgG-fractions and corresponding plasma samples from 17 children with type 1 diabetes and 23 unaffected children all testing negative for plasma IAA. The levels of IAA measured from IgG-fractions of diabetic children were higher than the levels of IAA measured from IgG-fractions in the control children (p = 0.004 in Mann-Whitney U-test). Forty-seven percent (8 out of 17) of newly-diagnosed patients negative for plasma IAA before IgG separation had increased levels of IAA in IgG-fractions and only 13% (3 out of 23) of controls. The levels of glutamate decarboxylase autoantibodies (GADA) did not differ between patients (n = 14) and controls (n = 21) negative for plasma GADA when measured in IgG-fractions. Our results suggest that formation of immune complexes results in false negative results in tests for IAA but not for GADA.

Place, publisher, year, edition, pages
2004. Vol. 37, no 1, 45-49 p.
Keyword [en]
IgG-fractions; immune complexes, insulin autoantibodies, type 1 diabetes
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-22086DOI: 10.1080/0887044032000158929Local ID: 1166OAI: diva2:242399
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2011-01-12

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Vaarala, Outi
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Faculty of Health SciencesPediatricsDepartment of Paediatrics in Linköping
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