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Loss of Smad3 in acute T-cell lymphoblastic leukemia
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2004 (English)In: New England Journal of Medicine, ISSN 0028-4793, Vol. 351, no 6, 552-559 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The receptors for transforming growth factor β (TGF-β) and their signaling intermediates make up an important tumor-suppressor pathway. The role of one of these intermediates - Smad3 - in the pathogenesis of lymphoid neoplasia is unknown. METHODS: We measured Smad3 messenger RNA (mRNA) and protein in leukemia cells obtained at diagnosis from 19 children with acute leukemia, including 10 with T-cell acute lymphoblastic leukemia (ALL), 7 with pre-B-cell ALL, and 2 with acute nonlymphoblastic leukemia (ANLL). All nine exons of the SMAD3 gene (MADH3) were sequenced. Mice in which one or both alleles of Smad3 were inactivated were used to evaluate the role of Smad3 in the response of normal T cells to TGF-β and in the susceptibility to spontaneous leukemogenesis in mice in which both alleles of the tumor suppressor p27Kip1 were deleted. RESULTS: Smad3 protein was absent in T-cell ALL but present in pre-B-cell ALL and ANLL. No mutations were found in the MADH3 gene in T-cell ALL, and Smad3 mRNA was present in T-cell ALL and normal T cells at similar levels. In mice, the loss of one allele for Smad3 impairs the inhibitory effect of TGF-β on the proliferation of normal T cells and works in tandem with the homozygous inactivation of p27Kip1 to promote T-cell leukemogenesis. CONCLUSIONS: Loss of Smad3 protein is a specific feature of pediatric T-cell ALL. A reduction in Smad3 expression and the loss of p27Kip1 work synergistically to promote T-cell leukemogenesis in mice.

Place, publisher, year, edition, pages
2004. Vol. 351, no 6, 552-559 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-22302DOI: 10.1056/NEJMoa031197Local ID: 1495OAI: diva2:242615
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2011-01-12

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Walz, Thomas
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Faculty of Health SciencesOncologyDepartment of Oncology UHL
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