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Mechanisms of cross-resistance between nucleoside analogues and vincristine or daunorubicin in leukemic cells
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Clinical Pharmacology.
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2004 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 320, no 3, 825-832 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to clarify the biochemical and molecular mechanisms behind the cross-resistance to nucleoside analogues (Nas) in four erythroleukemic cell lines with acquired resistance to the anthracycline daunorubicin and to the vinca alkaloid vincristine, expressing high levels of p-glycoprotein (P-gp, MDR1). All resistant strains exhibited cross-resistance to NA (cladribine and cytosine arabinoside) -induced apoptosis, assessed by caspase-3-like activation and were less sensitive to NA cytotoxicity in MTT assay. Real-time PCR and enzyme activity analysis showed reduced amounts of deoxycytidine kinase (35-80%) and elevated levels of 5′- nucleotidases (50-100%). The ratio 5′-nucleotidase to deoxycytidine kinase increased between 2.5- and 7.5-folds in resistant cells. This is in agreement with the observation that 5′-nucleotidase/ deoxycytidine kinase ratio might be an important factor in predicting resistance to Nas. Implications of this finding for combining anthracyclines or vinca alkaloids with Nas toward leukemic cells are discussed.

Place, publisher, year, edition, pages
2004. Vol. 320, no 3, 825-832 p.
Keyword [en]
cytosine arabinoside, arthracycline, p-glycoprotein, deoxycytidine kinase, 5-nucleotidase, cladribine
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-23410DOI: 10.1016/j.bbrc.2004.06.016Local ID: 2857OAI: diva2:243724
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2011-01-12

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Lotfi, Kourosh
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