Contribution of electron and confocal microscopy in the study of Leishmania-macrophage interactions
2004 (English)In: Microscopy and Microanalysis, ISSN 1431-9276, Vol. 10, no 5, 656-661 p.Article in journal (Refereed) Published
Promastigotes of the protozoan parasite genus Leishmania are inoculated into a mammalian host when an infected sand fly takes a bloodmeal. Following their opsonization by complement, promastigotes are phagocytosed by macrophages. There, promastigotes differentiate into amastigotes, the form of the parasite that replicates in the phagolysosomal compartments of host macrophages. Although the mechanisms by which promastigotes survive the microbicidal consequence of phagocytosis remain, for the most part, to be elucidated, evidence indicates that glycoconjugates play a role in this process. One such glycoconjugate is lipophosphoglycan, an abundant promastigote surface glycolipid. Using quantitative electron and confocal laser scanning microscopy approaches, evidence was provided that L. donovani promastigotes inhibit phagolysosome biogenesis in a lipophosphoglycan-dependent manner. This inhibition correlates with an accumulation of periphagosomal F-actin, which may potentially form a physical barrier that prevents L. donovani promastigote-containing phagosomes from interacting with endocytic vacuoles. Inhibition of phagosome maturation may constitute a strategy to provide an environment propitious to the promastigote-to-amastigote differentiation.
Place, publisher, year, edition, pages
2004. Vol. 10, no 5, 656-661 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-23684DOI: 10.1017/S1431927604040851Local ID: 3182OAI: oai:DiVA.org:liu-23684DiVA: diva2:243999