Modulation of Cytokine-Induced Production of IL-8 in Vitro by Interferons and Glucocorticosteroids
2004 (English)In: Inflammation, ISSN 0360-3997, E-ISSN 1573-2576, Vol. 28, no 2, 77-88 p.Article in journal (Refereed) Published
Interleukin-8 (IL-8) has been implicated in the pathogenesis of inflammation and cancer. Intracellular levels of cytokine-induced IL-8 in human umbilical vein endothelial cells (HUVEC) were modulated using interferons and steroids to further elucidate their mechanism. Basal and cytokine-induced production of IL-8 was studied using a novel ELISA application, flow cytometry, and RT-PCR. The intracellular amount of IL-8 increased after 6-h stimulation with TNF- (30%) or IL-1ß (55%) which was doubled when Golgi transport was disrupted using monensin. IFN-γ decreased the intracellular amount of IL-8 by 60% in both unstimulated and TNF--stimulated cells, but only when secretion was blocked using monensin. Dexamethasone inhibited the TNF--induced production by 33%, but had no effect in unstimulated cells. Our study indicated that both, dexamethasone and IFN inhibit TNF--induced upregulation of IL-8 at the mRNA level. It could be speculated that they inhibit IL-8 production by affecting different gene regulatory mechanisms.
Place, publisher, year, edition, pages
2004. Vol. 28, no 2, 77-88 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-24058DOI: 10.1023/B:IFLA.0000033023.76110.51Local ID: 3617OAI: oai:DiVA.org:liu-24058DiVA: diva2:244374