Aberrant regulation of interleukin-12 receptor β2 chain on type 1 cytokine-stimulated T lymphocytes in type 1 diabetes
2005 (English)In: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 114, no 2, 287-293 p.Article in journal (Refereed) Published
An aberrant mitogen-induced polarization of peripheral blood T cells has been associated with type 1 diabetes (T1D). We studied, in T1D, type 1 and 2 cytokine-induced expression of the interleukin-12 receptor β2 chain (IL-12Rβ2 chain), which plays a critical role in regulating T-cell polarization. Peripheral blood lymphocytes from children with newly diagnosed T1D (n = 10; mean age 10 years), from children with longstanding T1D (n = 8; mean age 12·9 years) and from healthy children (n = 15; mean age 11·5 years) were stimulated with phytohaemagglutinin (PHA) in a type 1 (IL-12 and anti-IL-4) or a type 2 (IL-4 and anti-IL-12) cytokine environment. Secretion of interferon-γ (IFN-γ), IL-5 and IL-13, as detected by enzyme-linked immunosorbent assay (ELISA), and expression of the IL-12Rβ2 chain on CD4 and CD8 cells by flow cytometry, were analysed. Children with newly diagnosed and longstanding T1D had lower expression levels of the IL-12Rβ2 chain on IL-12Rβ2 chain-positive CD4 T cells (for a type 1 or a type 2 cytokine environment: P = 0·01 and P = 0·002 or P = 0·02 and P = 0·01, respectively) and on IL-12Rβ2 chain-positive CD8 T cells (for a type 1 or a type 2 cytokine environment: P = 0·007 and P = 0·0007 or P = 0·003 and P = 0·01, respectively) when compared to healthy children. A decreased percentage of IL-12Rβ2 chain-expressing CD4 T cells (P = 0·07 and P = 0·03) and CD8 T cells (P = 0·004 and P = 0·01) and increased secretion of IL-13 (P = 0·006 and P = 0·04) in a type 1 cytokine environment was seen in both groups of patients. Peripheral blood T cells from patients with both newly diagnosed and longstanding T1D showed poor polarization towards type 1 cells.
Place, publisher, year, edition, pages
2005. Vol. 114, no 2, 287-293 p.
IL-12R, IL-13, T cells, type 1 diabetes
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-24403DOI: 10.1111/j.1365-2567.2004.02102.xLocal ID: 6500OAI: oai:DiVA.org:liu-24403DiVA: diva2:244721