Apoptosis in rectal carcinoma: Prognosis and recurrence after preoperative radiotherapy
2001 (English)In: Cancer, ISSN 0008-543X, E-ISSN 1097-0142, Vol. 91, no 10, 1870-1875 p.Article in journal (Refereed) Published
BACKGROUND: Rectal carcinoma is common, with considerable local recurrence and death rates. Preoperative radiotherapy and refined surgical techniques can improve local control. The aim of this study was to investigate the interaction between apoptosis and the outcome of rectal carcinoma, with and without short-term preoperative radiotherapy.
METHODS: Specimens were from 162 patients from the Southeast Swedish Health Care region included in the Swedish Rectal Cancer Trial between 1987-1990. New sections from the paraffin blocks of the preoperative biopsies and the surgical specimens were examined for apoptosis using the terminal deoxynucleotidyl transferase mediated digoxigenin nick end labeling (TUNEL) method.
RESULTS: The mean percentage of apoptotic cells was 0.3% (0-4%) and 1.1% (0-14.5%) for the preoperative biopsy and the surgical specimen, respectively. The authors analyzed the surgical specimens from nonirradiated patients and divided them into three groups by apoptotic index (AI) as follows: 0%, 0-1%, and > 1%. A high AI was associated with a decreased local recurrence rate compared with an intermediate or a low AI (P = 0.024). There was no significant relation between AI and survival. There was a significant reduction in the local recurrence rate for irradiated patients compared with the nonirradiated in the low (P = 0.015) and intermediate (P = 0.038) AI groups. In the high AI group, there were few recurrences and no significant difference was observed between irradiated and nonirradiated patients. The relative risk of death from rectal carcinoma in Dukes A-C patients was not significantly decreased by radiotherapy, but, in the intermediate AI group, there was a trend (P = 0.08) in favor of the irradiated patients.
CONCLUSION: A high AI in rectal carcinoma indicated a decreased local recurrence rate.
Place, publisher, year, edition, pages
2001. Vol. 91, no 10, 1870-1875 p.
apoptosis, rectal carcinoma, radiotherapy, local failure, disease-free survival
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-24825DOI: 10.1002/1097-0142(20010515)91:10<1870::AID-CNCR1208>3.0.CO;2-1Local ID: 9222OAI: oai:DiVA.org:liu-24825DiVA: diva2:245147