liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Immunolocalization of Cholecystokinin-2 Receptors in Rat Gastric Mucosa
Linköping University, Department of Biomedicine and Surgery, Cell biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Biomedicine and Surgery. Linköping University, Faculty of Health Sciences.
Department of Physiological Sciences, Section of Neuroendocrine Cell Biology, Lund University, Lund, Sweden.
Linköping University, Department of Biomedicine and Surgery. Linköping University, Faculty of Health Sciences.
Show others and affiliations
2002 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 37, no 9, 1017-1024 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin.

METHODS: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats I h before they were killed, the acid pump H,K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase.

RESULTS: Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H,K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors.

CONCLUSION: Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life.

Place, publisher, year, edition, pages
2002. Vol. 37, no 9, 1017-1024 p.
Keyword [en]
Cck2, Receptor, Gastrin, Immunofluorescence, Organogenesis, Progenitor Zone, Stomach
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-24956DOI: 10.1080/003655202320378194Local ID: 9367OAI: oai:DiVA.org:liu-24956DiVA: diva2:245280
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
In thesis
1. The role of gastrin and extracellular matrix proteins in proliferation and differentiation of gastric epithelial cells
Open this publication in new window or tab >>The role of gastrin and extracellular matrix proteins in proliferation and differentiation of gastric epithelial cells
2000 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The mechanisms regulating the proliferation and differentiation processes that give rise to and maintain the gastric epithelium have not yet been completely elucidated.

In the present studies, in vitro models were established and the influence of growth factors and extracellular matrix proteins on these processes were investigated. Pentagastrin and hydrocortisone were found to accelerate the development of H,KTPase-positive parietal cells and other epithelial cells from undifferentiated gastric epithelial cells of foetal rats. These undifferentiated cells and also presumably immature epithelial cells in the progenitor zone of adult gastric glands were shown to express cholecystokinin-2 (CCK2) receptors and are therefore targets for the trophic action of gastrin.

H,K-ATPase-positive parietal cells in the progenitor zone of adult glands were also found to express CCK2 receptors, indicating that gastrin may stimulate maturation of the parietal cell lineage even during adult life. Parietal cells located in the upper region of the glandular unit are probably responsible for most of the acid secretion, because these cells were found to express the membrane-cytoskeletallinker ezrin, reported to be present in the canaliculi of stimulated parietal cells.

Pulse-labelling with 5-bromo-2'-deoxyuridine showed that during the gestational period, when the main motphological conformations and maturation of the gastric epithelium occur, proliferating cells appear at the basal epithelial cell layer and migrate towards the gastric lumen. This indicates that epithelial-mesenchymal cell and cell-matrix interactions may be involved in regulation of the cell proliferation and differentiation. Investigation of the expression of extracellular matrix proteins in foetal rat stomachs revealed a marked increase in collagen type I, suggesting that collagen, which is known to stimulate epithelial cell proliferation, is involved in the initial folding of the embryonic epithelium and formation of glandular structures.

In experiments in vitro, development of mucus-producing cells from undifferentiated gastric epithelial cells was stimulated by collagens but inhibited by fibronectin and laminin. In adult gastric epithelium, collagen type I was present only in the pit region of the glandular unit, where surface mucous cells are located. Thus, collagen type I, which is overexpressed in gastric ulcers and gastric cancers, likely stimulates proliferation of mucus-producing cells.

In conclusion, expression of CCK2 receptors was detected in foetal gastric epithelium and in the progenitor zone of adult gastric epithelium, implying that gastrin exerts trophic effects on immature gastric epithelial cells, during both stomach organogenesis and adult life. The spatial and temporal expression of extracellular matrix proteins and the effects of these proteins on development of mucus-producing cells in vitro indicate that extracellular matrix proteins may play an important role in regulation of epithelial cell proliferation and differentiation, and thus in the maintenance of normal cellular composition and function ofthe gastric epithelium.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2000. 82 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 638
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-25719 (URN)10096 (Local ID)91-7219-739-0 (ISBN)10096 (Archive number)10096 (OAI)
Public defence
2000-09-22, Berzeliussalen, Universitetssjukhuset, Linköping, 13:00 (Swedish)
Opponent
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2012-11-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Borch, KurtMårdh, Sven

Search in DiVA

By author/editor
Borch, KurtMårdh, Sven
By organisation
Cell biologyFaculty of Health SciencesDepartment of Biomedicine and Surgery
In the same journal
Scandinavian Journal of Gastroenterology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf