Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysis
2000 (English)In: Journal of Medical Microbiology, ISSN 0022-2615, Vol. 49, no 9, 817-822 p.Article in journal (Refereed) Published
The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five histologically normal controls revealed a Helicobacter-specific band in the TTGE profile with increased amounts of Helicobacter-specific DNA in the biopsies from most of the gastritis patients. DNA from other genera including Enterococcus, Pseudomonas, Streptococcus, Staphylococcus and Stomatococcus was also found in the stomach. In the absence of gastric inflammation, Helicobacter spp. appeared to be part of a complex, presumably indigenous microbial flora found in the biopsy specimens from the stomach.
Place, publisher, year, edition, pages
2000. Vol. 49, no 9, 817-822 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-24957PubMedID: 10966230Local ID: 9368OAI: oai:DiVA.org:liu-24957DiVA: diva2:245281