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Suppressor genes for malignant and anchorage-independent phenotypes located on human chromosome 9 have no dosage effects
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Cell biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Cell biology.
2000 (English)In: Cytogenetics and Cell Genetics, ISSN 0301-0171, E-ISSN 1421-9816, Vol. 88, no 1-2, 103-109 p.Article in journal (Refereed) Published
Abstract [en]

We have previously shown that microcell-mediated transfer of a der(9)t(X,9) human chromosome (HSA), derived from human fibroblast strain GM0705, into the Syrian hamster cell line BHK-191-5C produced only near-tetraploid hybrids, although the recipient cell line contained a 1:1 ratio of near-diploid and near-tetraploid cells. However, the tumorigenicity and the anchorage independence could be suppressed in the near-tetraploid hybrids with one copy of the der(9)t(X,9) chromosome. The introduction of an HSA X chromosome did not suppress either of these phenotypes. We concluded that in addition to two suppressor genes, one for tumorigenicity and another for anchorage independence, HSA 9 might carry a third gene capable of inhibiting cellular growth in vitro, which had dosage effects. In the present study, keeping one copy of the der(9)t(X,9) chromosome, we have increased the hamster background chromosome number beyond hexaploid level by fusing two microcell-generated hybrid cell lines, where both malignant and anchorage-independent phenotypes were suppressed, with the parental malignant BHK-191-5C cell line. Tests with nude mice showed that hybrids containing one copy of the der(9)t(X,9) chromosome against the increased background of chromosomes of malignant parental origin were still suppressed for both phenotypes. These results suggest that the suppressor genes for malignancy and for anchorage independence have no dosage effects, in contrast to the suppressor gene(s) for cellular growth. Copyright (C) 2000 S. Karger AG, Basel.

Place, publisher, year, edition, pages
2000. Vol. 88, no 1-2, 103-109 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-25107Local ID: 9539OAI: oai:DiVA.org:liu-25107DiVA: diva2:245433
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13

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Islam, QuamrulIslam, Khaleda

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