Evidence for suppression of cellular growth in vitro and selection against the indigenous mouse X chromosome in A9 cell hybrids after microcell-modiated transfer of an X from other mammalian species
2000 (English)In: Cytogenetics and Cell Genetics, ISSN 0301-0171, Vol. 88, no 1-2, 110-113 p.Article in journal (Refereed) Published
Introduction of a human or Syrian hamster X chromosome (derived from BHK-191-5C cell hybrids) into tumorigenic mouse A9 cells via microcell fusion induced changes in cellular morphology and a retardation of cellular growth. The suppression of growth of the hybrids could be abolished, however, by daily changes of medium containing 20% serum. G-banding analysis showed the absence of a single, cytogenetically identifiable, indigenous X chromosome (marker Z) in two of four hybrid clones after an X chromosome was transferred from either hamster or human cells. All hybrids were tumorigenic when tested in nude mice. Together, these data suggest that the loss of the mouse X chromosome took place probably because of growth inhibitory effects imposed on hybrid cells due to the increase in X chromosome dosage. In addition, our results show a lack of association between the phenotype of cellular growth suppression in vitro and the phenotype of suppression of tumorigenicity in vivo. Copyright (C) 2000 S. Karger AG, Basel.
Place, publisher, year, edition, pages
2000. Vol. 88, no 1-2, 110-113 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25108Local ID: 9540OAI: oai:DiVA.org:liu-25108DiVA: diva2:245434