Tumor necrosis factor-alpha in ileal mast cells in patients with Crohn's disease
2000 (English)In: Digestion, ISSN 0012-2823, Vol. 61, no 1, 68-76 p.Article in journal (Refereed) Published
Background: Reports that both intestinal and extraintestinal Crohn's disease (CD) had healed successfully after treatment with anti-tumor necrosis factor-alpha (TNF-a) antibody have strengthened the hypothesis that it has a role in the treatment of CD. The macrophage is one source of TNF-a. Intestinal mast cells are also thought to have a role in CD, but it is not known if human ileal mast cells express TNF-a. Aim: To find out whether TNF-a is expressed by mast cells in the ileal wall in CD patients and controls. Methods: TNF-a was sought immunohistochemically in full thickness specimens of ileal wall from patients with CD (histologically normal, n = 9, inflamed, n = 6) and controls (patients with colonic cancer, n = 8). Mast cells were identified by metachromasia and anti-mast cell tryptase immunoreactivity. Results: In all layers of the ileal wall, and in every specimen investigated, mast cells were the main cell type that expressed TNF-a immunoreactivity out of the TNF-a-labelled cells. The number of TNF-a-labelled mast cells was greater in the muscularis propria in patients compared with controls, both in uninflamed (1.7-fold, p < 0.05) and in inflamed bowel (4.6-fold, p < 0.002), greater in the submucosa in inflamed compared with uninflamed CD (1.6-fold, p < 0.01), and less in the lamina propria in inflamed compared with uninflamed CD (0.4-fold, p < 0.05). Conclusion: Mast cells are an important source of TNF-a in all layers of the ileal wall, and the increased density of TNF-a-positive mast cells in the submucosa and muscularis propria may contribute to the tissue changes and symptoms in CD.
Place, publisher, year, edition, pages
2000. Vol. 61, no 1, 68-76 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25223DOI: 10.1159/000007737Local ID: 9662OAI: oai:DiVA.org:liu-25223DiVA: diva2:245550