Characterization of galactosyl glycerolipids in the HT29 human colon carcinoma cell line
2001 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, Vol. 396, no 2, 187-198 p.Article in journal (Refereed) Published
Glycoglycerolipids constitute a family of glycolipids with apparently very restricted expression in human tissues. They have previously been detected only in the testis and the nervous system. In the present study, two glycoglycerolipids were isolated from the HT29 human colon carcinoma cell line. The glycoglycerolipids were structurally characterized as a monogalactosylglycerolipid (1-O-alkyl-2-O-acyl-3-O-(▀-galactosyl)-sn-glycerol) and a digalactosylglycerolipid (1-O-alkyl-2-O-acyl-3-O-(▀-galactosyl(1-4)a-galactosyl)-sn- glycerol) using NMR and mass spectrometry. This digalactosylglycerolipid has not previously been structurally characterized. When HT29 cells were allowed to differentiate into more enterocyte-like cells by culture in glucose-free medium, expression of both of these glycoglycerolipids was greatly diminished. The presence of glycoglycerolipids in a human colon carcinoma cell line indicates that expression of this family of glycolipids may not be as restricted as previously thought. Instead this class of glycolipids may serve as differentiation antigens in various normal tissues and in tumor development. The Gala1-4Gal epitope was previously identified as a receptor for bacterial adhesins and toxins. The finding that this epitope is also linked to a glycerolipid moiety opens up new possible roles for this carbohydrate receptor in intracellular signaling.
Place, publisher, year, edition, pages
2001. Vol. 396, no 2, 187-198 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25319DOI: 10.1006/abbi.2001.2610Local ID: 9760OAI: oai:DiVA.org:liu-25319DiVA: diva2:245647