Apoptosis and Bcl-2 expression in relation to age, tumor characteristics and prognosis in breast cancer
1999 (English)In: International Journal of Biological Markers, ISSN 0393-6155, Vol. 14, no 2, 84-91 p.Article in journal (Refereed) Published
The extent of apoptosis and the expression of Bcl-2 was investigated in tumor samples from 165 women who underwent surgery for primary breast carcinoma between 1989 and 1990 in South-East Sweden. Apoptosis was assessed by a DNA fragmentation assay for flow cytometry. Bcl-2 protein expression was analyzed with immunocytochemistry. Bcl-2 immunoreactivity correlated with estrogen receptor (ER) and progesterone receptor (PgR) positivity and was inversely correlated with p53 accumulation. Apoptosis increased with patient age and a high degree of apoptosis was negatively associated with Bcl-2 immunostaining. Apoptosis showed no significant correlation with any of the other variables studied, including prognosis. The group with Bcl-2-positive tumors tended to have a lower risk of distant recurrence than others, but the association of Bcl-2 with recurrence was different in groups divided by ER and PgR status. Whereas Bcl-2 positivity indicated a low recurrence rate among PgR-negative patients, in the PgR-positive group, those with Bcl-2-positive tumors showed a non significantly higher recurrence rate than Bcl-2-negative cases. In the PgR-positive group, Bcl-2-positive tumors also appeared more frequently to be lymph node positive and DNA aneuploid. The results suggest that hormone receptor status is of importance for the prognostic role of Bcl-2. Likewise, patient age merits consideration when apoptosis is studied in human cancer.
Place, publisher, year, edition, pages
1999. Vol. 14, no 2, 84-91 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25386PubMedID: 10399627Local ID: 9829OAI: oai:DiVA.org:liu-25386DiVA: diva2:245715