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Acetazolamide inhibits stimulated feline liver and gallbladder bicarbonate secretion
Department of Surgery, Sahlgrenska University Hospital, Göteborg.
Centre of Hepatology, Gastroenterology and Dietetics, Clinic of Gastroenterology, Nephrourology and Surgery, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
Department of Transplantation and Liver Surgery, Sahlgrenska University Hospital, Göteborg,.
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2002 (English)In: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 174, no 2, 117-123 p.Article in journal (Refereed) Published
Abstract [en]

Bile acidification is a key factor in preventing calcium carbonate precipitation and gallstone formation. Carbonic anhydrase II (CA II), that is inhibited by acetazolamide, plays a role in regulation of the acid-base balance in many tissues. This study examines the effect of acetazolamide on secretin- and vasoactive intestinal peptide (VIP)-stimulated gallbladder mucosal bicarbonate and acid secretion. Gallbladders in anaesthetized cats were perfused with a bicarbonate buffer bubbled with CO2 in air. In 20 experiments VIP (10 ╡g kg1 h1) and in 10 experiments secretin (4 ╡g kg1 h1) were infused continuously intravenous (i.v.). Hepatic bile and samples from the buffer before and after perfusion of the gallbladder were collected for calculation of ion and fluid transport. During basal conditions a continuous secretion of H+ by the gallbladder mucosa was seen. Intravenous infusion of vasoactive intestinal peptide (VIP) and secretin caused a secretion of bicarbonate from the gallbladder mucosa (P < 0.01). This secretion was reduced by intraluminal (i.l.) acetazolamide (P < 0.01). Bile flow was enhanced by infusion of VIP and secretin (P < 0.01) but this stimulated outflow was not affected by i.v. acetazolamide. The presence of CA II in the gallbladder was demonstrated by immunoblotting. Biliary CA activity has an important function in the regulation of VIP- and secretin-stimulated bicarbonate secretion across the gallbladder mucosa.

Place, publisher, year, edition, pages
2002. Vol. 174, no 2, 117-123 p.
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Medical and Health Sciences
URN: urn:nbn:se:liu:diva-25394DOI: 10.1046/j.1365-201X.2002.00929.xPubMedID: 11860373Local ID: 9837OAI: diva2:245723
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2016-03-16Bibliographically approved

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Svanvik, Joar
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Faculty of Health SciencesSurgeryDepartment of Surgery in Östergötland
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