Activation of cAMP-dependent protein kinase is necessary for actin rearrangements in human neutrophils during phagocytosis
2000 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, Vol. 67, no 4, 520-528 p.Article in journal (Refereed) Published
We have investigated the role of cAMP and cAMP-dependent protein kinase (cAPK) in neutrophil phagocytosis. Inhibition of cAPK with H-89 reduced complement- and IgG-dependent phagocytosis to 83 and 46%, respectively. Fluorescence intensity measurements of phalloidin-stained actin in neutrophils showed a reduced amount of filamentous actin (F-actin) in pseudopods and around the phagosome in cells treated with H-89 or cAMP-elevating agents (forskolin and rolipram). The amount of phosphotyrosine-containing proteins was also reduced in pseudopods and around the phagosome. Taken together, the data show that cAMP/cAPK regulates F-actin reorganization during receptor-mediated phagocytosis, particularly triggered by IgG-FcR interaction. Our results support the hypothesis that active subcortical reorganization of F-actin is a prerequisite for FcR-mediated phagocytosis, but is less important during CR3-mediated ingestion.
Place, publisher, year, edition, pages
2000. Vol. 67, no 4, 520-528 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25401Local ID: 9844OAI: oai:DiVA.org:liu-25401DiVA: diva2:245730