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Activation of cAMP-dependent protein kinase is necessary for actin rearrangements in human neutrophils during phagocytosis
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Cell biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
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2000 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, Vol. 67, no 4, 520-528 p.Article in journal (Refereed) Published
Abstract [en]

We have investigated the role of cAMP and cAMP-dependent protein kinase (cAPK) in neutrophil phagocytosis. Inhibition of cAPK with H-89 reduced complement- and IgG-dependent phagocytosis to 83 and 46%, respectively. Fluorescence intensity measurements of phalloidin-stained actin in neutrophils showed a reduced amount of filamentous actin (F-actin) in pseudopods and around the phagosome in cells treated with H-89 or cAMP-elevating agents (forskolin and rolipram). The amount of phosphotyrosine-containing proteins was also reduced in pseudopods and around the phagosome. Taken together, the data show that cAMP/cAPK regulates F-actin reorganization during receptor-mediated phagocytosis, particularly triggered by IgG-FcR interaction. Our results support the hypothesis that active subcortical reorganization of F-actin is a prerequisite for FcR-mediated phagocytosis, but is less important during CR3-mediated ingestion.

Place, publisher, year, edition, pages
2000. Vol. 67, no 4, 520-528 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-25401Local ID: 9844OAI: oai:DiVA.org:liu-25401DiVA: diva2:245730
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2011-01-14

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Majeed, MeythamJ Rasmusson, BirgittaStendahl, OlleSärndahl, Eva

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