Formation of amyloid in human pancreatic islets transplanted to the liver and spleen of nude mice
2003 (English)In: Uppsala Journal of Medical Sciences, ISSN 0300-9734, Vol. 108, no 3, 193-203 p.Article in journal (Refereed) Published
In previous studies we have shown that apparently normal human islets, transplanted under the renal capsule of nude mice, frequently and rapidly develop amyloid deposits derived from the ▀-cell hormone islet amyloid polypeptide (IAPP). In the present study, we show for the first time that human islets, transplanted into the liver or spleen of nude mice, also develop islet amyloid rapidly. Ultrastructural studies of such islets showed that the first aggregation of IAPP takes place within the ▀-cells and that extracellular deposits show up later in the amyloid formation process. We also found that the amount of amyloid formed in human islet grafts placed under the kidney capsule increased with extended (26 weeks) observation time. Moreover, prolonged in vitro culture (14 days) prior to the implantation under the renal capsule seemed to enhance the formation of amyloid in the grafted islets. Since aggregated IAPP has been shown to be toxic to ▀-cells, the finding of amyloid deposits in transplanted islets offers a possible explanation to the frequent loss of function of islets transplanted into diabetic patients.
Place, publisher, year, edition, pages
2003. Vol. 108, no 3, 193-203 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25459Local ID: 9905OAI: oai:DiVA.org:liu-25459DiVA: diva2:245788