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Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, The Institute of Technology.
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, The Institute of Technology.ORCID iD: 0000-0001-6385-6760
Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Thoracic Surgery. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart Centre, Department of Thoracic and Vascular Surgery.
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2010 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 15, no 2Article in journal (Refereed) Published
Abstract [en]

Intramyocardial oxygen transport was assessed during open-chest surgery in calves by diffuse reflectance spectroscopy using a small intramuscular fiber-optic probe. The sum of hemo- and myoglobin tissue fraction and oxygen saturation, the tissue fraction and oxidation of cytochrome aa3, and the tissue fraction of methemoglobin, were estimated using a calibrated empirical light transport model. Increasing the oxygen content in the inhaled gas; 21%-50%-100%, in five calves (group A) gave an oxygen saturation of 19+/-4%, 24+/-5% and 28+/-8%, and mean tissue fractions of 1.6% (cytochrome aa3), and 1.1% (hemo- and myoglobin). Cardiac arrest in two calves gave an oxygen saturation lower than 5%. In two calves (group B) a left ventricular assistive device (LVAD pump) was implanted. Group B animals displayed similar trends in hemo- and myoglobin oxygen saturation as in group A, but at higher levels (maxima of 38% (B1) and 44% (B2)). The cytochrome aa3 oxidation level was above 96% in both group A and B calves, including the two cases involving cardiac arrest.

In conclusion, the estimated tissue fractions and oxygenation/oxidation levels of the myocardial chromophores during respiratory and hemodynamic provocations where in agreement with previously presented results, demonstrating the potential of the method.

Place, publisher, year, edition, pages
2010. Vol. 15, no 2
Keyword [en]
bio-optics, biotransport, blood vessels, cardiovascular system, diseases, fibre optic sensors, haemodynamics, molecular biophysics, muscle, oxidation, oxygen, prosthetics, proteins, reflectivity, respiratory protection, statistical analysis, surgery; Hemodynamics, Biomedical engineering, Fluid mechanics and rheology, Fluid transport and rheology, Pneumodyamics, respiration, Muscles
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-25585DOI: 10.1117/1.3374050ISI: 000278465300069OAI: oai:DiVA.org:liu-25585DiVA: diva2:246010
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2016-08-31Bibliographically approved
In thesis
1. Quantitative diffuse reflectance spectroscopy: myocardial oxygen transport from vessel to mitochondria
Open this publication in new window or tab >>Quantitative diffuse reflectance spectroscopy: myocardial oxygen transport from vessel to mitochondria
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In the field of biomedical optics, diffuse reflectance spectroscopy (DRS) is a frequently used technique for obtaining information about the optical properties of the medium under investigation. The method utilizes spectral difference between incident and backscattered light intensity for quantifying the underlying absorption and scattering processes that affects the light-medium interaction.

In this thesis, diffuse reflectance spectroscopy (DRS) measurements have been combined with an empirical photon migration model in order to quantify myocardial tissue chromophore content and status. The term qDRS (quantitative DRS) is introduced in the thesis to emphasize the ability of absolute quantification of tissue chromophore content. To enable this, the photon migration models have been calibrated using liquid optical phantoms. Methods for phantom characterization in terms of scattering coefficient, absorption coefficient, and phase function determination are also presented and evaluated. In-vivo qDRS measurements were performed on both human subjects undergoing routine coronary artery bypass grafting (CABG), and on bovine heart during open-chest surgery involving hemodynamic and respiratory provocations. The application of a hand-held fiber-optic surface probe (human subjects) proved the clinical applicability of the technique as the results were in agreement with other studies. However, problems with non-physiological variations in detected intensity due to intermittent probe-tissue discontact were observed. Also, systematic deviations between modeled and measured spectra were found. By model inclusion of additional chromophores revealing the mitochondrial oxygen uptake ability, an improved model fit to measured data was achieved. Measurements performed with an intramuscular probe (animal subjects) diminished the influence of probe-tissue discontact on the detected intensity. It was demonstrated that qDRS could quantify variations in myocardial oxygenation induced by physiological provocations, and that absolute quantification of tissue chromophore content could be obtained.

The suggested qDRS method has the potential of becoming a valuable tool in clinical practice, as it has the unique ability of monitoring both the coronary vessel oxygen delivery and the myocardial mitochondrial oxygen uptake ability. This makes qDRS suitable for directly measuring the result of different therapies, which can lead to a paradigm shift in the monitoring during cardiac anesthesia.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2009. 92 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1276
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-25587 (URN)978-91-7393-522-7 (ISBN)
Public defence
2009-10-30, Berzeliussalen, Campus US, Linköpings universitet, Linköping, 09:00 (English)
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Available from: 2009-10-15 Created: 2009-10-08 Last updated: 2016-08-31Bibliographically approved

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Lindbergh, TobiasLarsson, MarcusSzabó, ZoltánCasimir-Ahn, HenrikStrömberg, Tomas

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