Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves
2010 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 15, no 2Article in journal (Refereed) Published
Intramyocardial oxygen transport was assessed during open-chest surgery in calves by diffuse reflectance spectroscopy using a small intramuscular fiber-optic probe. The sum of hemo- and myoglobin tissue fraction and oxygen saturation, the tissue fraction and oxidation of cytochrome aa3, and the tissue fraction of methemoglobin, were estimated using a calibrated empirical light transport model. Increasing the oxygen content in the inhaled gas; 21%-50%-100%, in five calves (group A) gave an oxygen saturation of 19+/-4%, 24+/-5% and 28+/-8%, and mean tissue fractions of 1.6% (cytochrome aa3), and 1.1% (hemo- and myoglobin). Cardiac arrest in two calves gave an oxygen saturation lower than 5%. In two calves (group B) a left ventricular assistive device (LVAD pump) was implanted. Group B animals displayed similar trends in hemo- and myoglobin oxygen saturation as in group A, but at higher levels (maxima of 38% (B1) and 44% (B2)). The cytochrome aa3 oxidation level was above 96% in both group A and B calves, including the two cases involving cardiac arrest.
In conclusion, the estimated tissue fractions and oxygenation/oxidation levels of the myocardial chromophores during respiratory and hemodynamic provocations where in agreement with previously presented results, demonstrating the potential of the method.
Place, publisher, year, edition, pages
2010. Vol. 15, no 2
bio-optics, biotransport, blood vessels, cardiovascular system, diseases, fibre optic sensors, haemodynamics, molecular biophysics, muscle, oxidation, oxygen, prosthetics, proteins, reflectivity, respiratory protection, statistical analysis, surgery; Hemodynamics, Biomedical engineering, Fluid mechanics and rheology, Fluid transport and rheology, Pneumodyamics, respiration, Muscles
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25585DOI: 10.1117/1.3374050ISI: 000278465300069OAI: oai:DiVA.org:liu-25585DiVA: diva2:246010