DNA flow cytometry of cytokeratin selected breast cancer cells as prognostic indicator
1994 (English)Doctoral thesis, comprehensive summary (Other academic)
DNA flow cytometry is used for the estimation of cell proliferation (S-phase fraction) and abnormalities in cellular DNA content (DNA ploidy), parameters which yield prognostic information in breast carcinomas. However, stromal, inflammatory, and residual non-neoplastic epithelial cells overlap cancer cells in the DNA histogram and complicate the calculation of DNA ploidy and S-phase fraction. In the present thesis, the cell-specific intermediary filament of epithelial tissue, cytokeratin, has been used for flow cytometric selection of the epithelial cell population in order to reduce the influence from nonepithelial cells.
S-phase fractions with and without flow cytometric selection of cytokeratin-containing cells were analysed in 507 breast cancers. The association between S-phase fraction and distant recurrence was evaluated. Using univariate analysis, S-phase fraction of the epithelial cell population predicted recurrence more effectively than S-phase fraction from the unselected cell population. In multivariate analysis, S-phase fraction of the cytokeratin-containing population added significant prognostic infonnation to the S-phase from non-selected cells, tumor size, and hormone receptor content. Also, the cytokeratin method increased the ability to identify minor aneuploid populations, However, we obtained decreased separation of adjacent peaks in the DNA histogram, due to slightly increased coefficients of variation with the cytokeratin method. Also, there was a reduced proportion of cytokeratin positive cells registered with flow cytometry compared with immunohistochemistry. In experiments with endometrial carcinomas, S-phase estimates with and without selection of epithelial cells were closely correlated for cell populations with a high proportion of cancer cells but not for populations with a low proportion.
Place, publisher, year, edition, pages
Linköping: Linköpings universitet , 1994. , 49 p.
Linköping University Medical Dissertations, ISSN 0345-0082 ; 421
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25723Local ID: 10100ISBN: 91-7871-267-XOAI: oai:DiVA.org:liu-25723DiVA: diva2:246271
1994-05-11, Onkologens föreläsningssal, Universitetssjukhuset, Linköping, 09:00 (Swedish)
Fernö, Mårten, Docent
Papers, included in the Ph.D. thesis, are not registered and included in the posts from 1999 and backwards.2009-10-082009-10-082012-07-25Bibliographically approved