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White Blood Cell Subsets in Buffy Coat-Derived Platelet Concentrates: The Effect of Pre- and Poststorage Filtration
Linköping University, Department of Molecular and Clinical Medicine, Transfusion Medicine. Linköping University, Faculty of Health Sciences.
Department of Laboratory Medicine and Pathobiology, St. Michael's Hospital, Toronto, Canada.
Linköping University, Department of Molecular and Clinical Medicine, Transfusion Medicine. Linköping University, Faculty of Health Sciences.
2000 (English)In: Vox Sanguinis, ISSN 0042-9007, E-ISSN 1423-0410, Vol. 79, no 4, 235-241 p.Article in journal (Refereed) Published
Abstract [en]

Background and Objectives: Our objective was to study the effect of storage time on the filtration of platelet concentrates (PCs). We compared the total number of white blood cells (WBC), as well as the distribution of WBC subsets, in units filtered before and after storage.

Materials and Methods: Buffy coat-derived PCs were filtered either fresh or after 5 days of storage, and total WBC were enumerated by flow cytometry. WBC subsets were analyzed by flow cytometry with three-color fluorescence.

Results: The total number of white cells before filtration was significantly higher in fresh units compared with stored units, whereas in postfiltration samples the number of white cells was significantly lower in the fresh compared with the stored units. Although absolute numbers were significantly reduced, filtration also induced significant changes in the proportions of subsets in both fresh and stored units; the percentage of T cells was decreased, whereas the percentage of B cells and monocytes was increased after filtration.

Conclusion: Our results suggest that prestorage WBC filtration of platelet concentrates is superior in reducing the absolute numbers of WBC. However, both pre- and poststorage WBC filtration significantly affect the proportions of WBC in the final product, decreasing the number of T cells while apparently increasing the proportion of MHC class II-positive cell populations.

Place, publisher, year, edition, pages
2000. Vol. 79, no 4, 235-241 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-25802DOI: 10.1159/000056737Local ID: 10239OAI: oai:DiVA.org:liu-25802DiVA: diva2:246350
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13Bibliographically approved
In thesis
1. A study of factors influencing the quality of blood products during preparation, storage and filtration
Open this publication in new window or tab >>A study of factors influencing the quality of blood products during preparation, storage and filtration
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The quality of a cell concentrate to be transfused is dependent on the method of preparation and the storage conditions of the blood products. The aim of this study was to determine, compare and evaluate factors influencing the quality of platelet and erythrocyte concentrates. The influence of the method of preparation on platelet concentrates from whole blood and on leukocyte depletion by filtration of erythrocyte concentrates was studied. In addition, the influence of storage on leukocyte depletion by filtration of platelet and erythrocyte concentrates was investigated.

The method of preparation of platelet concentrates from whole blood influenced the release from the platelet α-granules. A significant increase in the release was found in the concentrates prepared from platelet-rich plasma compared with buffy coat. If the buffy coat was allowed to rest for <4 hours before centrifugation, this difference was significant until day 3 of storage. The ability of platelets to stimulate the growth of fibroblasts followed a similar course and decreased during preparation and storage.

The method of preparation of erythrocyte concentrates was shown to influence the outcome of leukocyte depletion by filtration. When hard spun, buffy coat depleted, concentrates were used, the number of leukocytes found in the filtrate was significantly higher compared with units that had been supplemented with an additional 5 or 10 ml of plasma. The flow rate during filtration and temperature of the unit was also shown to have an influence on the outcome on the number of leukocytes post filtration.

The storage time of both erythrocyte and platelet concentrates resulted in significant differences in the number ofleukocytes found after leukocyte depletion by filtration. A short storage time of erythrocyte concentrates was found to give a higher number of leukocytes after filtration compared with a longer storage time. This was in contrast to platelet concentrates where a filtration just after preparation, i.e. no storage time, gave better leukocyte depletion compared with 5 days of storage.

The distribution ofleukocyte subsets was also changed significantly by filtration. Comparing the pre- and post-filtration percentages of subsets in platelet concentrates, we found a lower percentage of T-lymphocytes and a higher percentage of B-lymphocytes and monocytes post filtration. In conclusion, the method of preparation of cell concentrates and the storage time have a substantial impact on the properties of the final product. Standardized and controlled procedures are of great importance in making optimal blood products.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2001. 64 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 667
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-28599 (URN)13753 (Local ID)91-7219-960-1 (ISBN)13753 (Archive number)13753 (OAI)
Public defence
2001-04-21, Elsa Brännströmssalen, Universitetssjukhuset, Linköping, 10:00 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-09-06Bibliographically approved

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Berlin, Gösta

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