Genosubtyping by sequencing group A, B and C meningococci: a tool for epidemiological studies of epidemics, clusters and sporadic cases
2000 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 108, no 7-8, 509-516 p.Article in journal (Refereed) Published
Genosubtyping, by sequencing variable regions (VRs) 1, 2 and 3 of the porA gene, was evaluated as a tool to detect clonality of isolates in meningococcal epidemics in Africa and clusters of disease in Sweden. All 63 examined meningococcal isolates were successfully genosubtyped. The isolates belonging to group A type 4 with genosubtype P1.20,9,35a showed little heterogeneity in African epidemics in 1988 and onwards. In Sweden, two meningococcal clones of group B type 15, with genosubtypes P1.7,16,35 and P1.7,16f,35, dominated during two clusters of meningococcal disease in 1995–96 and in sporadic cases thereafter. The characterisation of group C meningococci isolated during 1992 in Sweden indicated a cluster (type 2a with genosubtype P1.5a,10d,36b) connected with a discotheque visit. Two variants of VR2 (10p and 25b), not previously described, were found among the examined isolates. Nucleotide sequence analysis of VRs in the porA gene proved a valuable epidemiological tool since almost all isolates could be genosubtyped, in contrast to the phenotypic methods presently used.
Place, publisher, year, edition, pages
2000. Vol. 108, no 7-8, 509-516 p.
Epidemiology, genosubtyping, sequencing, Neisseria meningitidis, meningococcal disease
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25867DOI: 10.1034/j.1600-0463.2000.01087-8509.xLocal ID: 10304OAI: oai:DiVA.org:liu-25867DiVA: diva2:246415