Effects of lactoferrin on rat dermal interstitial fluid pressure (Pif) and in vitro endothelial barrier function
2001 (English)In: Acta Physiologica Scandinavica, ISSN 0001-6772, Vol. 171, no 4, 419-425 p.Article in journal (Refereed) Published
We recently demonstrated that intravenous (i.v.) injection of the iron-binding protein lactoferrin (Lf) followed by antilactoferrin (aLf) antibodies or iron-saturated Lf alone increased albumin extravasation in vivo in several tissues including skin. Increased driving pressure for blood-tissue exchange or direct effects of Lf on the endothelial barrier are possible mechanisms. We therefore, firstly, measured interstitial fluid pressure (Pif) in dermis of rats given 1 mg Lf i.v. followed 30 min later by aLf or saline and circulatory arrest 1 or 5 min thereafter and compared with controls. Secondly, transmonolayer passage of Evans blue labelled albumin (EB-albumin) was evaluated in porcine pulmonary artery endothelial cells exposed to iron-free or iron-saturated Lf (both 100 ╡g mL-1) in the absence and presence of 0.5 mM hydrogen peroxide. Pif increased significantly at 11-30 min following Lf to +2.1 ▒ 0.3 and +1.7 ▒ 0.2 mmHg at 11-20 and 21-30 min, respectively, compared with +0.1 ▒ 0.2 mmHg before Lf (P < 0.05, n = 25). Endothelial transmonolayer passage of EB-albumin during 3 h was not affected by iron-free or iron-saturated Lf neither in the absence nor presence of hydrogen peroxide that increased passage 3.5 times compared with controls. In conclusion, Lf-induced increase in albumin extravasation in rat skin is not explained by changes in Pif (because Lf raised Pif significantly) or direct effects of Lf on the endothelial barrier.
Place, publisher, year, edition, pages
2001. Vol. 171, no 4, 419-425 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25889Local ID: 10330OAI: oai:DiVA.org:liu-25889DiVA: diva2:246437