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Analysis of bacterial vaginosis-related amines in vaginal fluid by gas chromatography and mass spectrometry
Linköping University, Department of Molecular and Clinical Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Molecular and Clinical Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Molecular and Clinical Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
2001 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 39, no 11, 4026-4031 p.Article in journal (Refereed) Published
Abstract [en]

The presence of various amines in vaginal fluid from women with malodorous vaginal discharge has been reported before. The investigations have used several techniques to identify the amines. However, an optimized quantification, together with a sensitive analysis method in connection with a diagnostic procedure for vaginal discharge, including the syndrome of bacterial vaginosis, as defined by the accepted “gold standard,” has not been done before. We now report a sensitive gas chromatographic and mass spectrometric method for identifying the amines isobutylamine, phenethylamine, putrescine, cadaverine, and tyramine in vaginal fluid. We used weighted samples of vaginal fluid to obtain a correct quantification. In addition, a proper diagnosis was obtained using Gram-stained smears of the vaginal fluid that were Nugent scored according to the method of Nugent et al. (R. P. Nugent et al., J. Clin. Microbiol., 29:297–301, 1991). We found that putrescine, cadaverine, and tyramine occurred in high concentrations in vaginal fluid from 24 women with Nugent scores between 7 and 10. These amines either were not found or were found only in very low concentrations in vaginal fluid from women with Nugent scores of 0 to 3. There is a strong correlation between bacterial vaginosis and the presence of putrescine, cadaverine, and tyramine in high concentrations in vaginal fluid.

Place, publisher, year, edition, pages
2001. Vol. 39, no 11, 4026-4031 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-25913DOI: 10.1128/JCM.39.11.4026-4031.2001ISI: 000171934200034Local ID: 10355OAI: oai:DiVA.org:liu-25913DiVA: diva2:246461
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13
In thesis
1. Amines and Bacterial Vaginosis
Open this publication in new window or tab >>Amines and Bacterial Vaginosis
2002 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Bacterial Vaginosis (BY) is a common syndrome, with a prevalence of 10-30% in women of childbearing age. The decisive pathogenetic factoris thought to have a microbiological origin, but so far no specific bacteria have been implicated in causing BV. Instead, it appears that BV is accompanied by a shift in the normal lactobacilli flora to a mixed vaginal anaerobic flora. Vaginal fluid from women with BY has also been reported to contain various amines, and several techniques have been used to identify these amines.

We developed a sensitive gas chromatographic and mass spectrometric (GC-MS) method to analyze amines related to BV together with quantification of the amines isobutylamine, phenethylamine, putrescine, cadaverine and tyramine. The aim of our investigation was to study if the amine content in vaginal fluids is quantitatively related to BV, diagnosed according to the Nugent scoring system. Our results show that the production of putrescine, cadaverine and tyramine is a property of BV, and that samples from healthy women do not include these amines.

Using a sensitive gas chromatographic method, we also analyzed and quantified vaginal fluids with respect to trimethylamine (TMA), the amine responsible for the fishy odor in BV. In order to obtain a proper identification of BV, the vaginal fluid samples were Gram-stained and diagnosed according to two procedures. Our results show that regardless of the scoring method used for diagnosis, vaginal fluids from women with BV generally contain elevated amounts of TMA, while samples from healthy women do not.

In conclusion, the presence of specific amines is clearly a prominent finding in women with BV, and these amines can thus be used as selective markers or diagnostic tools for the syndrome.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2002. 36 p.
Series
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 56
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-26684 (URN)11251 (Local ID)91-7373-184-6 (ISBN)11251 (Archive number)11251 (OAI)
Presentation
2013-09-20, Hälsouniversitetet, Linköping, 13:00 (Swedish)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2013-09-18

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Wolrath, HelenForsum, UrbanLarsson, Per-GöranBorén, Hans

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