Apolipoprotein A-1-derived amyloid in atherosclerotic plaques of the human aorta
2001 (English)In: Journal of Pathology, ISSN 0022-3417, E-ISSN 1096-9896, Vol. 193, no 2, 270-275 p.Article in journal (Refereed) Published
Previous studies have shown that the amyloid localized to the aortic intima may be a biochemical entity different from other forms of localized amyloid. The amyloid fibril protein in one patient studied consisted of an N-terminal fragment of apolipoprotein A-1 (apo A-1). Since this patient was later shown to carry a missense mutation in the apo A-1 gene, leading to a deletion at position 107 of the mature protein, the question remained whether wild-type apo A-1 is amyloidogenic. In autopsy specimens from the thoracic aorta from 69 individuals, intimal atherosclerotic plaque-related amyloid was present in 11 cases (16%) and amyloid outside plaques in 37 cases (54%). The immunoreactivity of amyloid localized to the aortic intima was evaluated with the aid of antisera against N-terminal segments of apo A-1. The amyloid in association with atherosclerotic plaques was positively labelled by immunohistochemistry. The amyloid fibril protein from one patient, previously shown not to carry any mutation in the apo A-1 gene, was purified and shown by amino acid sequence analysis to be of apo A-1 nature. The result shows that wild-type apo A-1 is amyloidogenic and gives rise to a common localized form of amyloid associated with atherosclerosis.
Place, publisher, year, edition, pages
2001. Vol. 193, no 2, 270-275 p.
amyloid, aorta, intima, apolipoprotein A-1, atherosclerosis, immunocytochemistry
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-25966DOI: 10.1002/1096-9896(2000)9999:9999<::AID-PATH753>3.0.CO;2-SLocal ID: 10414OAI: oai:DiVA.org:liu-25966DiVA: diva2:246514