liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Probing 23S ribosomal RNA cleavage sites in coccoid Helicobater pylori.
Linköping University, Faculty of Health Sciences. Linköping University, Department of health and environment.
Linköping University, Faculty of Health Sciences. Linköping University, Department of health and environment. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Microbiology.
2001 (English)In: Helicobacter, ISSN 1083-4389, Vol. 6, no 2, 100-109 p.Article in journal (Refereed) Published
Abstract [en]

Background. Previous studies have revealed that extensive nonrandom fragmentation of ribosomal RNA occurs during conversion of Helicobacter pylori to the coccoid form. The 16S rRNA fragmentation has been characterised in some detail. The aim of the present study was to define corresponding cleavage-sites in the 3'-half of the 23S rRNA molecule. Materials and Methods. Northern blot analysis using 23S rRNA specific antisense riboprobes and a 5'-end- labelled oligonucleotide probe was used to analyse the 23S rRNA fragmentation pattern in coccoid H. pylori type strain CCUG 17874T and H. pylori 26695, for which the genome has been sequenced. A double- stranded cDNA-dependent (ds-cDNA) primer- extension analysis technique using 23S rRNA ds-cDNA and a primer targeting the vicinity of the peptidyl-transferase centre was used to determine cleavage sites at the nucleotide level. Results. We report here the mapping of putative cleavage sites within domains IV and V, enclosing the peptidyl transferase centre, in the 3'-half of the 23S rRNA molecule. Three cleavage sites were located in domain IV. Two other cleavage sites were located in the peptidyl transferase centre, and one presumptive multiple-break site between helices 77 and 78 in domain V. The DNA motifs were different from the postulated A + U rich single-strand cleavage sites recognised by RNase E, which has been implicated in rRNA degradation in Escherichia coli. Conclusions. The present analysis suggests that a hitherto unknown mechanism is responsible for the nonrandom fragmentation of rRNA in coccoid H. pylori, which may have important consequences for the growth, and survival of the bacterium.

Place, publisher, year, edition, pages
2001. Vol. 6, no 2, 100-109 p.
Keyword [en]
23S rRNA cleavage sites, Coccoid Helicobacter pylori, Ds-cDNA primer extension
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-25978DOI: 10.1046/j.1523-5378.2001.00015.xLocal ID: 10427OAI: oai:DiVA.org:liu-25978DiVA: diva2:246526
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2009-11-01

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Monstein, Hans-JurgJonasson, Jon

Search in DiVA

By author/editor
Monstein, Hans-JurgJonasson, Jon
By organisation
Faculty of Health SciencesDepartment of health and environmentDepartment of Clinical Microbiology
In the same journal
Helicobacter
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 44 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf