PCR Identification of the Group A Neisseria Meningitidis Gene in Cerebrospinal Fluid
1999 (English)In: Scandinavian Journal of Infectious Diseases, ISSN 0036-5548, Vol. 31, no 5, 481-483 p.Article in journal (Refereed) Published
The aim of this study was to develop a PCR method for direct identification of Neisseria meningitidis serogroup A in cerebrospinal fluid. The assay makes use of unique sites within the gene cassette responsible for expression of the (α1→6)-linked N-acetyl-D-mannosamine-1-phosphate serogroup A capsule. A total of 67 different N. meningitidis strains and 12 clinical samples of CSF, culture positive for N. meningitidis, were examined. All the strains and samples of N. meningitidis serogroup A were correctly identified by an amplified PCR product of 519 bp. The PCR method for identification is specific for the group A gene of N. meningitidis. The assay may contribute to reducing recurrent, devastating epidemics of meningococcal infection by providing a diagnostic tool for grouping in developing countries where problems with false negative cultures are common and vaccination against serogroup A meningococci may be required.
Place, publisher, year, edition, pages
1999. Vol. 31, no 5, 481-483 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26071DOI: 10.1080/00365549950164003Local ID: 10530OAI: oai:DiVA.org:liu-26071DiVA: diva2:246619