Involvement of the ß2-integrin CD18 in apoptosis signal transduction in human neutrophils
2000 (English)In: Inflammation Research, ISSN 1023-3830, E-ISSN 1420-908X, Vol. 49, no 9, 452-459 p.Article in journal (Refereed) Published
Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β2-integrin activation, and further investigate the signal transduction pathways involved.
Material: Human polymorphonuclear neutrophils.
Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).
Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.
Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p < 0.01), and activate the production of reactive oxygen species (p < 0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.
Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.
Place, publisher, year, edition, pages
2000. Vol. 49, no 9, 452-459 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26104DOI: 10.1007/s000110050616Local ID: 10563OAI: oai:DiVA.org:liu-26104DiVA: diva2:246652