Increased IFN-? response to transplantation antigens measured by cytokine MLC: Indications for a bi-phasic response pattern
2003 (English)In: Transplant Immunology, ISSN 0966-3274, Vol. 11, no 1, 101-105 p.Article in journal (Refereed) Published
The immunological reactivity to major histocompatibility transplantation antigens (MHC) is important in clinical manifestations such as graft rejection and graft-vs.-host disease. To evaluate the allogeneic cytokine response pattern we used a newly developed cytokine mixed leukocyte culture (MLC) technique. Blood mononuclear cells from healthy women (n=6) were exposed to cells from another person and to pooled cells from 28 blood donors (MHC-pool). The secretion kinetics of IL-4 and IFN-? from the responder cells was analysed by ELISPOT. We found a higher IFN-? response to the MHC-pool compared with the IL-4 secretion. Both the total secretion of IFN-? for 7 days and the median value of IFN-? in each individual was increased compared with IL-4. The IFN-? response showed a bi-phasic pattern with the major peak on day 6-7. Our results indicate that allo-responses are mainly Thl-like responses, displaying a bi-phasic pattern. This knowledge may be useful, and the methods suitable, in the studies of allo-responses in transplantation and pregnancy.
Place, publisher, year, edition, pages
2003. Vol. 11, no 1, 101-105 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26418DOI: 10.1016/S0966-3274(02)00146-6Local ID: 10960OAI: oai:DiVA.org:liu-26418DiVA: diva2:246967