Pulsed-field gel electrophoresis as an epidemiologic tool for Neisseria gonorrhoeae: Identification of clusters within serovars
2002 (English)In: Sexually Transmitted Diseases, ISSN 0148-5717, E-ISSN 1537-4521, Vol. 29, no 1, 25-31 p.Article in journal (Refereed) Published
Background: The increasing incidence of gonorrhea in Sweden in 1998 was due to mostly domestic cases. Among these, two core groups were identified: homosexual men with serovar IB-2 and young heterosexuals with serovar IB-3.
Goals: To explore the genetic homogeneity/heterogeneity within the predominant serovars, IB-2 and IB-3, of Neisseria gonorrhoeae in Sweden by pulsed-field gel electrophoresis (PFGE) and to compare these results to epidemiologic information, as well as examine the genetic diversity within and between the 25 other represented serovars of N gonorrhoeae.
Study Design: By PFGE, 237 N gonorrhoeae isolates were examined, and the results were compared with epidemiologic data for the IB-2 and IB-3 isolates.
Results: In 79% of the domestic IB-2 cases involving homosexuals and 66% of the domestic IB-3 cases involving young heterosexuals, the isolates were genetically indistinguishable by PFGE. A high genetic diversity was identified within and between the 27 included serovars.
Conclusions: Examination by means of PFGE indicated that one N gonorrhoeae clone each of the serovars IB-2 and IB-3 created the majority of the two core groups of domestic cases.
Place, publisher, year, edition, pages
2002. Vol. 29, no 1, 25-31 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26457PubMedID: 11773875Local ID: 11005OAI: oai:DiVA.org:liu-26457DiVA: diva2:247006