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The cell clinic: closable microvials for single cell studies
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-2071-7768
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
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2002 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 4, no 3, 177-187 p.Article in journal (Refereed) Published
Abstract [en]

We present the development of a cell clinic. This is a micromachined cavity, or microvial, that can be closed with a lid. The lid is activated by two polypyrrole/Au microactuators. Inside the microvials two Au electrodes have been placed in order to perform impedance studies on single or a small number of cells. We report on impedance measurements on Xenopus leavis melanophores. We could measure a change in the impedance upon cell spreading and identify intracellular events such as the aggregation of pigment granules. The electrical data is correlated to optical microscopy.

Place, publisher, year, edition, pages
2002. Vol. 4, no 3, 177-187 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-26486DOI: 10.1023/A:1016092228965Local ID: 11042OAI: oai:DiVA.org:liu-26486DiVA: diva2:247035
Note

The original publication is available at www.springerlink.com: Edwin WH Jager, Charlotte Immerstrand, Kajsa Holmgren Peterson, Karl-Eric Magnusson, Ingemar Lundström and Olle Inganäs, The cell clinic: Closable microvials for single cell studies, 2002, Biomedical microdevices (Print), (4), 3, 177-187. http://dx.doi.org/10.1023/A:1016092228965 Copyright: Springer Verlag (Germany) http://www.springerlink.com/

Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13
In thesis
1. Biophysical studies of pigment transport in frog melanophores: impedance measurements and advanced microscopy analyses
Open this publication in new window or tab >>Biophysical studies of pigment transport in frog melanophores: impedance measurements and advanced microscopy analyses
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Small proteins, other molecules and large organelles are frequently transported from one location to another within cells. These transports employ cytoskeletal networks and enable cells to maintain regions with different functions and attain an asymmetrical shape.

The aim of this work was to explore biophysical methods for monitoring intracellular transport processes and associated structural changes. For these studies we have used pigment cells, melanophores, from the African clawed frog Xenopus laevis. In response to external stimuli, these cells can change colour by redistributing pigment granules in the cytoplasm.

Transparent "cell clinics" equipped with gold electrodes were developed for impedance studies. The results show that impedance measurements at different frequencies not only can be used to monitor cell attachment and spreading, but also events like pigment aggregation. Significant F-actin breakdown and a cell area decrease may explain the impedance decrease seen during latrunculin-induced aggregation. In aggregation induced by melatonin there was, however, a small increase of the cell area, no F-actin breakdown and still lowered impedance, indicating that some other, likely intracellular mechanism is involved. In addition, confocal laser scanning microscopy (CLSM) studies showed that aggregation was associated with an increase in the cell height, more prominent for latrunculin than for melatonin. This height increase did not seem to involve influx of water through aquaporin channels at the cell membrane, or newly formed or remodelled microtubules in the cells.

Besides impedance measurements, Image Correlation Spectroscopy (ICS) was applied to analyse pigment aggregation. The study shows for the first time that ICS can be used to analyse aggregation of non-fluorescent particles and suggests that the method may provide new information on the state of aggregation of granules in pigment cells.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2003. 58 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 803
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-26657 (URN)11222 (Local ID)91-7373-491-8 (ISBN)11222 (Archive number)11222 (OAI)
Public defence
2003-09-26, Victoriasalen, Hälsouniversitetet, Linköping, 09:00 (Swedish)
Opponent
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2012-10-17Bibliographically approved

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Jager, Edwin W.H.Immerstrand, CharlotteHolmgren Peterson, KajsaMagnusson, Karl-EricLundström, IngemarInganäs, Olle

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Jager, Edwin W.H.Immerstrand, CharlotteHolmgren Peterson, KajsaMagnusson, Karl-EricLundström, IngemarInganäs, Olle
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