Distinct Effects of Protein Kinase C on the Barrier Function at Different Developmental Stages
2003 (English)In: Bioscience Reports, ISSN 0144-8463, E-ISSN 1573-4935, Vol. 23, no 2-3, 87-102 p.Article in journal (Refereed) Published
We show here, that activation of protein kinase C by the phorbol ester PMA improves barrier function in colon carcinoma (HT 29) cells. By contrast, in canine kidney (MDCK I) cells it caused increased permeability and opening of tight junctions; the latter has also been noticed in other studies. Thus, with PMA confluent HT 29 cells responded with a reduced passage of 330 kDa sodium fluorescein, increased transepithelial electrical resistance, and a change in the cell shape of the HT 29 cells from an irregular to a regular, hexagonal form. Confocal imaging revealed parallel distinct changes in the staining of occludin and caludin-1, viz. a translocation from cytoplasmic clusters to apical cell–cell contacts. Interestingly, in both cell lines protein kinase A activation caused a decreased in the threonine phosphorylation of occludin that correlated with tight junction assembly in HT 29 cells and tight junction disassembly in MDCK I cells. We conclude that protein kinase C regulation of the epithelial barrier involves specific molecular mechanisms and achieves distinct effects at different developmental stages.
Place, publisher, year, edition, pages
2003. Vol. 23, no 2-3, 87-102 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26487DOI: 10.1023/A:1025524323842ISI: 000185101500004Local ID: 11043OAI: oai:DiVA.org:liu-26487DiVA: diva2:247036