liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Effect of heat denaturation on beta-lactoglobulin-induced gastrointestinal sensitization in rats: denatured betaLG induces a more intensive local immunologic response than native betaLG
Show others and affiliations
2002 (English)In: Pediatric Allergy and Immunology, ISSN 0905-6157, Vol. 13, no 4, 269-277 p.Article in journal (Refereed) Published
Abstract [en]

Beta-lactoglobulin (▀LG) is one of the first foreign antigens encountered by a newborn child, and it is the major allergen causing cow's milk allergy. Heat denaturation causes changes to the protein structure, but the significance of heat-induced changes for immunogenicity or allergenicity is not known. To clarify how heat denaturation affects allergenicity and immunogenicity, we immunized Hooded-Lister rat pups with intra-peritoneal injections of native or heat-denatured ▀LG at days 43 and 62 after birth. The animals were then fed native and denatured milk products twice weekly from 73 to 101 days of age with a feeding tube, after which they were allowed cheese and milk ad libitum, until they were killed on day 131. Total immunoglobulin (Ig)E and ▀LG-specific IgG1 and IgG2a levels were determined from serum samples. Spontaneous interleukin-4 (IL-4) and interferon-? (IFN-?) production was measured from duodenal specimens, and specimens of gastrointestinal mucosae were studied for the presence of inflammatory cells. The rats immunized with native ▀LG had higher levels of total serum IgE than the unimmunized controls or the rats immunized with heat-denatured ▀LG, while heat-denatured ▀LG induced a significantly more intensive mononuclear inflammatory cell and eosinophil infiltration in the gastroduodenal mucosa. The ▀LG -specific IgG antibody and IL-4 and IFN-? responses were similar in the two groups of immunized animals. Hence, denaturation modifies the immunogenic and allergenic properties of ▀LG. Heat-denatured ▀LG induces a more intensive local reaction in the gastrointestinal mucosa, while there is some evidence for enhanced systemic allergic sensitization by native ▀LG. ⌐ 2002 Blackwell Munksgaard.

Place, publisher, year, edition, pages
2002. Vol. 13, no 4, 269-277 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-26500DOI: 10.1034/j.1399-3038.2002.01028.xLocal ID: 11056OAI: diva2:247049
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-13

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Jenmalm, Maria
By organisation
Faculty of Health SciencesPediatrics
In the same journal
Pediatric Allergy and Immunology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 32 hits
ReferencesLink to record
Permanent link

Direct link