liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effect of heat denaturation on beta-lactoglobulin-induced gastrointestinal sensitization in rats: denatured betaLG induces a more intensive local immunologic response than native betaLG
Show others and affiliations
2002 (English)In: Pediatric Allergy and Immunology, ISSN 0905-6157, Vol. 13, no 4, 269-277 p.Article in journal (Refereed) Published
Abstract [en]

Beta-lactoglobulin (▀LG) is one of the first foreign antigens encountered by a newborn child, and it is the major allergen causing cow's milk allergy. Heat denaturation causes changes to the protein structure, but the significance of heat-induced changes for immunogenicity or allergenicity is not known. To clarify how heat denaturation affects allergenicity and immunogenicity, we immunized Hooded-Lister rat pups with intra-peritoneal injections of native or heat-denatured ▀LG at days 43 and 62 after birth. The animals were then fed native and denatured milk products twice weekly from 73 to 101 days of age with a feeding tube, after which they were allowed cheese and milk ad libitum, until they were killed on day 131. Total immunoglobulin (Ig)E and ▀LG-specific IgG1 and IgG2a levels were determined from serum samples. Spontaneous interleukin-4 (IL-4) and interferon-? (IFN-?) production was measured from duodenal specimens, and specimens of gastrointestinal mucosae were studied for the presence of inflammatory cells. The rats immunized with native ▀LG had higher levels of total serum IgE than the unimmunized controls or the rats immunized with heat-denatured ▀LG, while heat-denatured ▀LG induced a significantly more intensive mononuclear inflammatory cell and eosinophil infiltration in the gastroduodenal mucosa. The ▀LG -specific IgG antibody and IL-4 and IFN-? responses were similar in the two groups of immunized animals. Hence, denaturation modifies the immunogenic and allergenic properties of ▀LG. Heat-denatured ▀LG induces a more intensive local reaction in the gastrointestinal mucosa, while there is some evidence for enhanced systemic allergic sensitization by native ▀LG. ⌐ 2002 Blackwell Munksgaard.

Place, publisher, year, edition, pages
2002. Vol. 13, no 4, 269-277 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-26500DOI: 10.1034/j.1399-3038.2002.01028.xLocal ID: 11056OAI: oai:DiVA.org:liu-26500DiVA: diva2:247049
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-13

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Jenmalm, Maria

Search in DiVA

By author/editor
Jenmalm, Maria
By organisation
Faculty of Health SciencesPediatrics
In the same journal
Pediatric Allergy and Immunology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 48 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf