Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments
2002 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, Vol. 110, no 3, 263-272 p.Article in journal (Refereed) Published
The rapid identification of the etiological agent of microbial infections can bring about both clinical and financial benefits. Thus, fast and generally applicable classification methods are needed that will enable us to rapidly distinguish pathogenic bacteria from commensals or saprophytic bacteria found in the same habitat. We here show that provisional classification of bacterial isolates can be performed on a large scale based on 16S rRNA sequence comparisons using PyrosequencingÖ, a recently described real-time DNA sequence analysis technique, and the concept of signature matching. The probes we have developed, together with the new technology, will enable early diagnosis of specific pathogens, which is critical for the rational use of antimicrobial therapy in clinical medicine.
Place, publisher, year, edition, pages
2002. Vol. 110, no 3, 263-272 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26501DOI: 10.1034/j.1600-0463.2002.100309.xLocal ID: 11058OAI: oai:DiVA.org:liu-26501DiVA: diva2:247050