Characterization of Human Alpha-Dystrobrevin Isoforms in HL-60 Human Promyelocytic Leukemia Cells Undergoing Granulocytic Differentiation
2002 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 13, no 12, 4195-4205 p.Article in journal (Refereed) Published
The biochemical properties and spatial localization of the protein alpha-dystrobrevin and other isoforms were investigated in cells of the human promyelocytic leukemia line HL-60 granulocytic differentiation as induced by retinoic acid (RA). Alpha-dystrobrevin was detected both in the cytosol and the nuclei of these cells, and a short isoform (gamma-dystrobrevin) was modified by tyrosine phosphorylation soon after the onset of the RA-triggered differentiation. Varying patterns of distribution of alpha-dystrobrevin and its isoforms could be discerned in HL-60 promyelocytes, RA-differentiated mature granulocytes, and human neutrophils. Moreover, the gamma-dystrobrevin isoform was found in association with actin and myosin light chain. The results provide new information about potential involvement of alpha-dystrobrevin and its splice isoforms in signal transduction in myeloid cells during induction of granulocytic differentiation and/or at the commitment stage of differentiation or phagocytic cells.
Place, publisher, year, edition, pages
2002. Vol. 13, no 12, 4195-4205 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26511DOI: 10.1091/mbc.E02-03-0128Local ID: 11068OAI: oai:DiVA.org:liu-26511DiVA: diva2:247060
On the day of the defence day the status of this article was submitted.2009-10-082009-10-082012-09-17Bibliographically approved