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Helicobacter pylori sabA adhesin in persistent infection and chronic inflammation
Department of Odontology/Oral Microbiology, Umeå University, Umeå, Sweden.
Department of Odontology/Oral Microbiology, Umeå University, Umeå, Sweden.
Department of Odontology/Oral Microbiology, Umeå University, Umeå, Sweden.
Department of Odontology/Oral Microbiology, Umeå University, Umeå, Sweden and The Swedish Institute for Infectious Disease Control, Solna, Sweden.
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2002 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 297, no 5581, 573-578 p.Article in journal (Refereed) Published
Abstract [en]

Helicobacter pylori adherence in the human gastric mucosa involves specific bacterial adhesins and cognate host receptors. Here, we identify sialyl-dimeric-Lewis x glycosphingolipid as a receptor for H. pylori and show thatH. pylori infection induced formation of sialyl-Lewis x antigens in gastric epithelium in humans and in a Rhesus monkey. The corresponding sialic acid–binding adhesin (SabA) was isolated with the “retagging” method, and the underlyingsabA gene (JHP662/HP0725) was identified. The ability of many H. pylori strains to adhere to sialylated glycoconjugates expressed during chronic inflammation might thus contribute to virulence and the extraordinary chronicity of H. pylori infection.

Place, publisher, year, edition, pages
2002. Vol. 297, no 5581, 573-578 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-26539DOI: 10.1126/science.1069076Local ID: 11100OAI: oai:DiVA.org:liu-26539DiVA: diva2:247088
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Characterisation of surface traits of Helicobacter pylori and their role in the infectious process
Open this publication in new window or tab >>Characterisation of surface traits of Helicobacter pylori and their role in the infectious process
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The surface appendages of bacteria determine the initial contact with host cells. Characterisation of functional organisation and spatial distribution of adhesive traits of outer membrane components of Gram-negative bacteria is a key issue in studies of the parasite-host cell interaction.

With focus on the enteropathogenic Helicobacter pylori, evidenced to cause chronic gastric infections in humans, detergent-digested freeze fracture replica labelling was applied for ultrastructural analyses of envelope distribution of the virulence factors blood group antigen binding adhesin (BabA), and the carbonic anhydrases (α-CA, ß-CA). In a preliminary study the methodology was also used to study the bacteria-host contact between phagocytosing human neutrophils and wild-type H. pylori.

In parallel, bacterial traits were analysed from a molecular and biochemical perspective. This included the specific roles of the BabA and the sialic acid-binding adhesin (SabA), and the neutrophil activating protein (HP-NAP) in neutrophilic stimulation and subsequent inflammatory process. It was concluded that SabA is crucial in the initiation of a neutrophilic response in the mediated inflammation.

This thesis has demonstrated the synergistic application of ultrastructural, molecular and cellular microbiology tools for delineating complex patterns in bacteria-host interactions, thus utilising the well-characterised and clinically important human pathogen H. pylori. This approach could be applicable to other Gram-negative species to clarify known and discern new virulence mechanisms in the multifaceted field of bacterial pathogenesis and bacterial interactions with human host cells.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2003. 77 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 805
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-26665 (URN)11231 (Local ID)91-7373-490-X (ISBN)11231 (Archive number)11231 (OAI)
Public defence
2003-10-03, Elsa Brändströmsalen, Hälsouniversitetet, Linköping, 09:00 (Swedish)
Opponent
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2012-10-15Bibliographically approved

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Petersson, ChristofferMagnusson, Karl-Eric

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