Immunologic activity in the small intestinal mucosa of pediatric patients with type 1 diabetes
2003 (English)In: Diabetes, ISSN 0012-1797, Vol. 52, no 9, 2287-2295 p.Article in journal (Refereed) Published
Involvement of gut immune system has been implicated in the pathogenesis of type 1 diabetes. However, few studies have been performed on the gut mucosa from patients with type 1 diabetes. Thus, we characterized the stage of immune activation in jejunal biopsy samples from 31 children with type 1 diabetes by immunohistochemistry, in situ hybridization, and RT-PCR. We found enhanced expressions of HLA-DR, HLA-DP, and intercellular adhesion molecule-1 by immunohistochemistry even on structurally normal intestine of patients with type 1 diabetes and no signs of celiac disease. In addition, the densities of IL-1a- and IL-4-positive cells detected by immunohistochemistry and IL-4 mRNA-expressing cells evaluated by in situ hybridization were increased in the lamina propria in patients with type 1 diabetes and normal mucosa. Instead, the densities of IL-2, ?-interferon (IFN-?), and tumor necrosis factor a-positive cells, the density of IFN-? mRNA positive cells, and the amounts of IFN-? mRNA detected by RT-PCR correlated with the degree of celiac disease in patients with type 1 diabetes. Our study supports the hypothesis that a link exists between the gut immune system and type 1 diabetes.
Place, publisher, year, edition, pages
2003. Vol. 52, no 9, 2287-2295 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26602DOI: 10.2337/diabetes.52.9.2287Local ID: 11167OAI: oai:DiVA.org:liu-26602DiVA: diva2:247151