On-line extraction using an alkyl-diol silica precolumn for racemic citalopram and its metabolites in plasma: results compared with solid-phase extraction methodology
2001 (English)In: Journal of Chromatography B: Biomedical Sciences and Applications, ISSN 1387-2273, Vol. 753, no 2, 365-373 p.Article in journal (Refereed) Published
Sample preparation is usually the most critical and time consuming step when using HPLC for drug analysis in biological matrixes. Sample extracts have to be clean considering both chromatographic interferences and column maintenance. To meet some of these criteria a fully automated on-line extraction (OLE) analysis method was developed for the antidepressant drug citalopram and its two demethylated metabolites, using an RP-C4-ADS extraction column. A comparison between the new OLE method and an off-line solid-phase extraction method showed that the two methodologies were equal in analytical precision but that the OLE method was faster and therefore superior in sample capacity per day.
Place, publisher, year, edition, pages
2001. Vol. 753, no 2, 365-373 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-26834DOI: 10.1016/S0378-4347(00)00579-XLocal ID: 11449OAI: oai:DiVA.org:liu-26834DiVA: diva2:247384