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13C NMR spectroscopy studies of forest soil microbial activity: Glucose uptake and fatty acid biosynthesis
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Radio Physics. Östergötlands Läns Landsting, Centre of Surgery and Oncology, Department of Radiation Physics.ORCID iD: 0000-0001-8661-2232
2001 (English)In: Soil Biology and Biochemistry, ISSN 0038-0717, Vol. 33, no 4-5, 621-632 p.Article in journal (Refereed) Published
Abstract [en]

The intimate association of soil microorganisms with the soil matrix complicates analysis of their metabolism, since thorough separation of intact cells from the matrix is very difficult using standard protocols. Thus, in the study reported here, in situ glucose decomposition and metabolism in humus from a coniferous forest soil was monitored and evaluated using 'solution state' 13C NMR, which can be used in a non-invasive manner. [U-13C] glucose was added at a concentration of 1.73 mmol C g-1 dry organic matter, which is known to allow maximal substrate induced respiration (SIR), and the microbial metabolism of the added C was followed over a period of 28 days. The data showed that ~50% of the added glucose was consumed within three days, coinciding with the appearance of label in CH3, -CH2- and -CH = CH-groups, and in glycerol-carbons, suggesting that olefinic triacylglycerols were being formed, probably located in oil droplets. During days two to three, around 40% of the consumed glucose C was allocated into solid state components, about 40% was respired and about 20% was found as triglycerols. The triacylglycerol signal reached a maximum after 13 days, but subsequently declined by 60%, as the triacylglycerols were apparently consumed, by day 28 of the incubation. Our results indicate there was an initial formation of structural microbial C (solid state carbon) followed by formation of storage lipid C, which subsequently decreased, probably because it was used to provide the organisms with energy when the external energy source (i.e. the glucose) was depleted. The formation of unsaturated triacylglycerols, typical storage metabolites of eucaryotes, suggests that fungi were the most active organisms in the glucose degradation. ⌐ 2001 Elsevier Science Ltd.

Place, publisher, year, edition, pages
2001. Vol. 33, no 4-5, 621-632 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-27014DOI: 10.1016/S0038-0717(00)00206-6Local ID: 11656OAI: diva2:247565
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2014-10-02

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Lundberg, Peter
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Faculty of Health SciencesRadio PhysicsDepartment of Radiation Physics
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