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Experimental transplantation of autologous iris pigment epithelial cells to the subretinal space
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL/MH.
2001 (English)In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, Vol. 79, no 5, 509-514 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: To investigate the cellular morphology in the subretinal space following transplantation of iris pigment epithelial (IPE) cells from the same eye. Methods: Following an iridectomy, fresh IPE cells were prepared and no culturing performed. After pars plana vitrectomy, a suspension of autologous IPE cells was injected into the subretinal space in 37 rabbits. The grafts were monitored by ophthalmoscopy and colour fundus photography. Rabbits were sacrificed at 1, 2, 3 and 6 months, respectively, and the eyes examined with light and electron microscopy. Results: The grafted area retained the same configuration over 6 months but then appeared less pigmented. At 1-3 months, the IPE formed one or more contiguous layers on top of native RPE. At 6 months, cells compatible with grafted IPE were present in the subretinal space, often forming monolayer-like chains integrating with the native RPE. Depigmented cells of presumed IPE origin were seen and frequently in association with abundant melanin granules located in the apical portion of adjacent RPE cells. In such areas, large macrophage-like cells were observed. Conclusion: Transplanted IPE cells survived for up to 6 months in the subretinal space. Our observations suggest a scenario of remodelling of the cellular layers in the subretinal space over time where grafted IPE cells formed a compound layer with the native RPE. Transplantation of autologous IPE cells may have a potential as a treatment modality in selected cases of age-related macular degeneration.

Place, publisher, year, edition, pages
2001. Vol. 79, no 5, 509-514 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-27599DOI: 10.1034/j.1600-0420.2001.790517.xLocal ID: 12329OAI: oai:DiVA.org:liu-27599DiVA: diva2:248151
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-13

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Crafoord, SvenAlgvere, Peep

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Faculty of Health SciencesOphthalmologyDepartment of Ophthalmology UHL/MH
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