Induction of cell death by the lysosomotropic detergent MSDH
2000 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 470, no 1, 35-39 p.Article in journal (Refereed) Published
Controlled lysosomal rupture was initiated in lysosome-rich, macrophage-like cells by the synthetic lysosomotropic detergent, O-methyl-serine dodecylamide hydrochloride (MSDH). When MSDH was applied at low concentrations, resulting in partial lysosomal rupture, activation of pro-caspase-3-like proteases and apoptosis followed after some hours. Early during apoptosis, but clearly secondary to lysosomal destabilization, the mitochondrial transmembrane potential declined. At high concentrations, MSDH caused extensive lysosomal rupture and necrosis. It is suggested that lysosomal proteases, if released to the cytosol, may cause apoptosis directly by pro-caspase activation and/or indirectly by mitochondrial attack with ensuing discharge of pro-apoptotic factors.
Place, publisher, year, edition, pages
2000. Vol. 470, no 1, 35-39 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-27689DOI: 10.1016/S0014-5793(00)01286-2Local ID: 12427OAI: oai:DiVA.org:liu-27689DiVA: diva2:248241