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Dynamic Ca2+ changes in neutrophil phagosomes. A source for intracellular Ca2+ during phagolysosome formation?
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Pathology. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Pathology and Clinical Genetics.
2000 (English)In: Cell Calcium, ISSN 0143-4160, Vol. 27, no 6, 353-362 p.Article in journal (Refereed) Published
Abstract [en]

An increase in cytosolic Ca2+ concentration periphagosomally is critical for phagolysosomal formation and neutrophil elimination of microbes. The Ca2+ increase could be achieved through release of Ca2+ from mobilized intracellular stores. Alternatively, Ca2+ that passively enter the phagosome during phagocytosis could be provided by the phagosome. Intraphagosomal Ca2+ changes in single human neutrophils was measured during phagocytosis of serum opsonized Fura-2-conjugated zymosan particles, using a digital image processing system for microspectrofluorometry. A decrease in phagosomal Ca2+ down to nanomolar concentrations was seen within minutes following phagosomal closure. Blockage of plasma membrane Ca2+ channels by econazole abolished this decrease. The fluorescence properties of Fura-2 zymosan were retained after phagocytosis and stable to pH changes, reactive oxygen species, and proteolytic enzymes. We suggest that Ca2+ ions present in the phagosome enter the cell cytosol through Ca2+ channels in the phagosomal membrane, achieving a localized Ca2+ rise that is important for phagosome processing.

Place, publisher, year, edition, pages
2000. Vol. 27, no 6, 353-362 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-27858DOI: 10.1054/ceca.2000.0130Local ID: 12618OAI: diva2:248410
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-14

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Lundqvist-Gustafsson, Helen
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Faculty of Health SciencesPathologyDepartment of Clinical Pathology and Clinical Genetics
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